畜牧与兽医2025，Vol.57Issue(1)：50-56,7.
BNIP3L介导的线粒体自噬在镉致大鼠大脑皮质神经元凋亡中的作用
The role of BNIP3L-mediated mitophagy in cadmium-induced apoptosis in rat cerebral cortical neurons
摘要
Abstract
In order to determine the regulatory effect of BNIP3L-mediated mitophagy on apoptosis induced by cadmium(Cd),the rat cere-bral cortical neuron BNIP3L gene silencing model was established,in this study,by RNA interference technique,and then the model was treated with 10μmol/L Cd for 12 h.Next,the expression levels of BNIP3L,Parkinson disease protein 2(Parkin)and mitochondrial apopto-sis pathway-related proteins cleaved caspase-9 and cleaved caspase-3 were detected by Western blot.The co-localization of BNIP3L and the mitochondrial marker protein TOMM20 was detected by immunofluorescence staining.And,transmission electron microscopy was used to determine the number of mitophagosomes in neurons.Finally,apoptosis was detected by FITC Annexin V staining.The results showed that the expression of BNIP3L was significantly increased(P＜0.01),and the co-localization of BNIP3L and TOMM20 was increased after Cd treatment for 12 h.Silencing BNIP3L significantly inhibited Cd-induced Parkin translocation to mitochondria(P＜0.01),inhibited Cd-in-duced mitophagosome formation,and significantly promoted Cd-induced caspase-9 and caspase-3 activation and neuronal apoptosis(P＜0.01).The present results indicated that BNIP3L-mediated mitophagy was able to inhibit Cd-induced apoptosis in rat cerebral cortical neurons.关键词
镉/大鼠大脑皮质神经元/BNIP3L/线粒体自噬/凋亡Key words
cadmium/rat cerebral cortical neuron/BNIP3L/mitophagy/apoptosis分类
畜牧业引用本文复制引用
闻双全,王亮,方兰,袁燕..BNIP3L介导的线粒体自噬在镉致大鼠大脑皮质神经元凋亡中的作用[J].畜牧与兽医,2025,57(1):50-56,7.基金项目
江苏省自然科学基金项目(BK20221372) （BK20221372）
江苏省高等学校基础科学研究面上项目(24KJB330009,22KJB360016) （24KJB330009,22KJB360016）
博士启动资金项目(2023) （2023）
太仓基础研究项目(TC2022JC30) （TC2022JC30）
高等学校学科创新引智计划资助项目(D18007) （D18007）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
