畜牧与兽医2025，Vol.57Issue(1)：79-85,7.
5-氨基乙酰丙酸抗猪繁殖与呼吸综合征病毒作用的研究
Study on anti-PRRSV effect of 5-aminolevulinic acid
摘要
Abstract
Porcine reproductive and respiratory syndrome(PRRS)is an acute,highly contagious disease caused by porcine reproductive and respiratory syndrome virus(PRRSV).5-aminolevulinic acid(5-ALA),as a natural amino acid,plays important antiviral function,and it inhibits replication of multiple viruses.However,whether 5-ALA can exert anti-PRRSV effect has not been reported,to our knowledge.In this study,the cytotoxicity of 5-ALA on PAM-KNU cells was detected by CCK-8 assay.The optimal method for and the optimal concentra-tion of 5-ALA to inhibit PRRSV propagation were determined by three modes of administration:prevention,direct killing and treatment.The results showed that the viral titers and cDNA copies were significantly down-regulated when the 5-ALA concentration was 1 mmol/L.Mean-while,the anti-PRRSV effect of prevention and direct killing of 5-ALA were more effective than the function of treatment.The effect of 5-ALA on PRRSV replication cycle was investigated by three periods:adsorption,internalization and early intracellular replication.The results showed that 5-ALA played a key role in the internalization and early intracellular replication period to inhibit PRRSV replication.Our study suggested that 5-ALA could inhibit PRRSV replication,which might serve as theoretical support for the prevention and treatment of PRRSV infection.关键词
猪繁殖与呼吸综合征病毒/5-氨基乙酰丙酸/抗病毒Key words
porcine reproductive and respiratory syndrome virus/5-aminolevulinic acid/antiviral effect分类
畜牧业引用本文复制引用
王凯月,杜以军,姜丹丹,郑宇航,李均同,吴香菊,胡悦,齐静,丛晓燕,孙英峰..5-氨基乙酰丙酸抗猪繁殖与呼吸综合征病毒作用的研究[J].畜牧与兽医,2025,57(1):79-85,7.基金项目
山东省自然科学基金项目(ZR2021MC139,ZR2023MC076) （ZR2021MC139,ZR2023MC076）
国家自然科学基金项目(32102710) （32102710）
国家兽用生物制品工程技术研究中心开放课题项目(GTKF(23)009) （GTKF(23）
