畜牧与兽医2025，Vol.57Issue(1)：111-118,8.
猪繁殖与呼吸综合征病毒谱系8毒株特异性单克隆抗体研制
Development of monoclonal antibodies specific to the lineage 8 strains of porcine reproductive and respiratory syndrome virus
摘要
Abstract
Porcine reproductive and respiratory syndrome virus(PRRSV),which is a pathogen that seriously endangers China's breeding industry,has multiple genotypes(lineages)and antigenic differences in circulating strains.In order to identify the circulating strains of dif-ferent lineages,the NSP2 recombinant protein was prepared by using the NSP2 hypervariable region gene fragment(tNSP2)of the PRRSV lineage 8 epidemic strain BB0907(a highly pathogenic strain),and the NSP2 recombinant protein obtained was used to immunize BALB/c mice.As a result,five monoclonal cell lines that could stably secrete antibodies were developed by cell fusion.The titers of the antibodies in ascites fluid in mice were ≥1∶216 000.The heavy chains of 3D6,6B2 and 6C8 were IgG1,the heavy chain of 8C2 was IgG2,while the heavy chain of 8H6 was IgG2a,and the light chains of the five McAbs were of kappa subtype.Four epitopes were identified by the construc-tion of NSP2 protein truncates,which were485GPLNFPTPSE494,555FPLAPSQNMG564,575EVLSEISDIL584 and 585NDTPAPVS593.These mono-clonal antibodies were observed to be able to react specifically with 8 strains of the PRRSV lineage by indirect immunofluorescence assay,but they did not react with the lineage 1 and lineage 5 strains.This study providedsignificantresearch materials for the differential diagnosis of and biological research onisolated PRRSV antigens.关键词
猪繁殖与呼吸综合征病毒/Nsp2/单克隆抗体/B细胞表位Key words
porcine reproductive and respiratory syndrome virus/nonstructural protein 2/monoclonal antibody/B cell epitopes分类
农业科技引用本文复制引用
任语馨,张杰,高雁怩,孙杨杨,张路捷,白娟,姜平..猪繁殖与呼吸综合征病毒谱系8毒株特异性单克隆抗体研制[J].畜牧与兽医,2025,57(1):111-118,8.基金项目
江苏省自主创新研发基金项目(CX(22)1011) （CX(22）
国家生猪产业技术体系专项(CARS-35) （CARS-35）
国家自然科学基金项目(32230103) （32230103）
