湖北科技学院学报（医学版）2025，Vol.39Issue(1)：1-5,5.DOI:10.16751/j.cnki.2095-4646.2024052306
芹菜素上调Quaking表达减轻棕榈酸诱导心肌细胞凋亡
Apigenin Alleviates Palmitic Acid-induced Cardiomyocytes Apoptosis by Upregulating Quaking Expression
摘要
Abstract
Objective To explore the role and mechanism of apigenin(APi)in palmitate acid(PA)-induced apoptosis in H9C2 cardiomyocytes.Methods APi(or QKI overexpression)intervened in PA-induced apoptosis of H9C2 cardiomyocytes;West-ern Blot was performed to detect protein expression in cells;Intracellular calcium concentration was determined by Fluo-4AM.Results 10μmol/L APi had the best effect on PA-induced Cleaved-caspase 3 protein expression in H9C2 cardiomyocytes,and the inhibitory effect was gradually weakened as the concentration of APi increased.The intervention of APi significantly inhibited the PA-induced down-regulation of QKI,SERCA2α,RyR2 expression and up-regulation of Cleaved-caspase 3 protein expression.Simil-ary,overexpression of QKI significantly inhibited the PA-induced down-regulation of QKI,SERCA2α,RyR2 expression and up-reg-ulation of Cleaved-caspase 3 protein expression.The intervention of APi(or QKI overexpression)alleviated the PA-induced in-crease in calcium concentration in H9C2 cardiomyocytes.Conclusion APi promotes the expression of QKI in cardiomyocytes,there-by maintaining intracellular Ca2+dynamic balance and improving PA-induced cardiomyocyte apoptosis.关键词
芹菜素/QKI/棕榈酸/钙离子/细胞凋亡Key words
Apigenin/QKI/Mitochondria/Calcium ion/Apoptosis分类
药学引用本文复制引用
杨雅元,阚洪爽,贾宏玉,杨晓松,欧阳昌汉,李莹莹,王淼..芹菜素上调Quaking表达减轻棕榈酸诱导心肌细胞凋亡[J].湖北科技学院学报（医学版）,2025,39(1):1-5,5.基金项目
国家自然科学基金面上项目(82073852) （82073852）
咸宁市自然科学基金(2021ZRKX036,2023ZRKX086) （2021ZRKX036,2023ZRKX086）
