中国农业大学学报2025，Vol.30Issue(2)：40-50,11.DOI:10.11841/j.issn.1007-4333.2025.02.04
葡萄VvWRKY53的分子特性与表达模式分析
Molecular characteristics and expression pattern analysis of grapevine VvWRKY53
摘要
Abstract
To explore the biological function of VvWRKY53,the VvWRKY53 gene containing a coding sequence(CDS)of 453 bp was cloned by RT-PCR from the cDNA of'Thompson seedless'.The molecular characteristics of the VvWRKY53 gene and its encoded protein were analyzed using bioinformatics tools.The subcellular localization of candidate protein was determined by transient expression in Nicotiana benthamiana.The expression patterns of the VvWRKY53 gene after spraying with four hormones:salicylic acid(SA),abscisic acid(ABA),methyl jasmonate(MeJA),and 1-aminocyclopropane-1-carboxylic acid(ACC);two elicitors:chitin and flg22,and after inoculation with Lasiodiplodia theobromae were detected using quantitative real-time PCR(qRT-PCR).The transcriptional activation or inhibitory activity of VvWRKY53 was determined by yeast system.The results showed that:The molecular weight of the VvWRKY53 protein is 18.1 ku,which has a theoretical isoelectric point(pl)of 9.67.The VvWRKY53 protein is located in the nucleus of Nicotiana benthamiana.The phylogenetic tree analysis indicates that VvWRKY53 has high similarity to AtWRKY45 and AtWRKY75 proteins.The results of qRT-PCR reveal that the relative expression level of VvWRKY53 changed after spraying SA,MeJA and ACC on'seedless white'grape leaves.The relative expression level of VvWRKY53 gene is gradually increased after ABA treatment.Both chitin and flg22 can rapidly induce the expression of the gene.The relative expression of VvWRKY53 gene continued to be up-regulated after inoculation of'summer black'semi-woody branches with Lasiodiplodia theobromae.It is confirmed that VvWRKY53 has transcriptional activation activity in yeast.The results indicate that the transcription factor VvWRKY53 may play an important role in the process of Lasiodiplodia theobromae infection.关键词
葡萄/可可毛色二孢/WRKY转录因子/生物信息学分析/基因表达Key words
grapevine/Lasiodiplodia theobromae/WRKY transcription factor/bioinformatics analysis/gene expression分类
农业科技引用本文复制引用
董梦迪,吴佳鸿,周悦妍,郝博雯,张玮,彭军波,邢启凯..葡萄VvWRKY53的分子特性与表达模式分析[J].中国农业大学学报,2025,30(2):40-50,11.基金项目
国家现代农业产业技术体系资助项目(CARS-29) （CARS-29）
国家自然科学基金项目(31801686) （31801686）
北京市农林科学院科技创新能力建设项目(KJCX20190406) （KJCX20190406）
