中国兽医杂志2025，Vol.61Issue(1)：7-13,7.DOI:10.20157/j.cnki.zgsyzz.2025.01.002
9种犬圆形细胞肿瘤鉴别诊断方法的建立和应用
Establishment and Application of Differential Diagnostic Methods for Nine Types of Canine Round Cell Tumor
摘要
Abstract
The differential diagnosis of canine round cell tumor(RCT)poses a significant challenge in veterinary pathology,with commercially available diagnostic kits often being imported,expensive,or inaccessible.To address this issue,this study utilized staining analyses involving 18 immunomarker antibodies and one specific dye targeting nine types of canine RCTs,optimizing the staining protocols.The results identified 13 effective immunomarker antibodies and one dye,including ionized calcium binding adapter molecule 1(Iba 1)for histiocytomas,cluster of differentiation 3(CD3)for T-cell lymphomas,cluster of differentiation 20(CD20)for B-cell lymphomas,and toluidine blue(T-blue)for mast cell tumors.Using these optimized staining methods,14 clinical canine RCT cases were successfully diagnosed,demonstrating reliable diagnostic outcomes.This study provides a cost-effective antibody combination and staining protocol for the differential diagnosis of canine RCTs.关键词
犬/圆形细胞肿瘤(RCT)/免疫组织化学/免疫标志物/鉴别诊断Key words
canine/round cell tumor(RCT)/immunohistochemistry/immunomarker/differential diagnosis分类
畜牧业引用本文复制引用
张竞,马莹,甄理,刘政,魏建伟,贺文琦,陆慧君..9种犬圆形细胞肿瘤鉴别诊断方法的建立和应用[J].中国兽医杂志,2025,61(1):7-13,7.基金项目
吉林省科技发展计划项目(20230508002RC) （20230508002RC）
