安徽农业科学2025，Vol.53Issue(2)：71-76,6.DOI:10.3969/j.issn.0517-6611.2025.02.017
斜带石斑鱼源的哈维氏弧菌分离与鉴定
Isolation and Identification of Vibrio harveyi from Epinephelus coioides
摘要
Abstract
[Objective]To identify the pathogen that caused deaths of Epinephelus coioides in a farm in Zhanjiang,conducted the dissection of Epinephelus coioides with white raised scabies on skin,and bacterial isolation and purification.[Method]Though biochemical reactions,16S rRNA gene cloning and sequencing,a phylogenetic tree was constructed to identify the dominant strain S1.The growth curve of V.harveyi S1 was plotted by OD600 and plate viable count.According to R2,it would determine the correlation and reliability of its growth curve.[Result]The iden-tification result showed that S1 is one of Vibrio harveyi,the same as V.harveyi hq-V149 converged into one branch.The drug sensitivity test re-sults showed that Vibrio harveyi S1 was sensitive to 12 antibiotics such as norfloxacin,cephalexin,levofloxacin and ciprofloxacin,but not to 12 antibiotics such as penicillin G,amtreonam,streptomycin,cefazolin and cefoperazone.[Conclusion]Vibrio harveyi S1 is the pathogen which caused deaths of Epinephelus coioides in a farm in Zhanjiang.The exponential growth time of S1 is 5-12 hours,and then enters the plateau peri-od.关键词
哈维氏弧菌/16S rRNA/斜带石斑鱼/药敏试验/生长曲线Key words
Vibrio harveyi/16S rRNA/Epinephelus coioides/Drug sensitivity test/Growth curve分类
农业科技引用本文复制引用
徐露茜,顾豪铭,许馨兰,汪志文,鲁义善..斜带石斑鱼源的哈维氏弧菌分离与鉴定[J].安徽农业科学,2025,53(2):71-76,6.基金项目
深圳市科技计划项目(JCYJ20210324130014035). （JCYJ20210324130014035）
