安徽农业科学2025，Vol.53Issue(2)：77-83,7.DOI:10.3969/j.issn.0517-6611.2025.02.018
MS-222对异育银鲫(Carassius auratus gibelio)麻醉复苏效果及抗氧化酶活性的影响
Effects of MS-222 on Anesthesia Recovery and Antioxidant Enzyme Activity of Carassius auratus gibelio
摘要
Abstract
In order to investigate the effects of MS-222 on anesthetic level,recovery rate and antioxidant enzyme activity of Carassius auratus gibelio(5.88±0.78 g),seven different concentrations of MS-222(50,60,70,80,90,100 and 110 mg/L)were set.The optimal anesthesia concentration and time of MS-222 to C.gibelio were determined based on the anesthesia stage and time,activities of antioxidant enzymes in the gill and mRNA expression of antioxidant-related genes.The result showed that C.gibelio could enter the stage Ⅰ and stage Ⅱ of anesthesia while anaesthetized at concentrations of 60,70,80,90 and 100 mg/L,and could recover within 10 min.C.gibelio could not enter deep anesthesia while the anesthetic concentration lower than 50 mg/L,and the cumulative mortality was 40％ while the concentration higher than 100 mg/L.Furthermore,two concentrations of MS-222 at 60 mg/L and 100 mg/L were set,and C.gibelio exposed for different time to study the oxidative damage of MS-222 to gills.Results showed that deep anesthesia with 100 mg/L MS-222(about 4 min),GSH activity and MDA content in gills of C.gibelio were significantly increased(P<0.05).The mRNA relative expression of HSP70,keap1,PIK3A1,nrf1,MAPK3 and caspase3 were significantly up-regulated(P<0.05).The POD activity,and mRNA relative expression of caspase3 and LYZ in the gills were recovered to the basal level after 6 h of recovery.After deep anesthesia with 60 mg/L MS-222(about 24 h),GSH activity in gills increased and POD de-creased significantly(P<0.05).The transcription of genes NQO,keap1,PIK3A1,Nrf1,MAPK3 and caspase3 were significantly up-regulated(P<0.05).After 1 min of recovery within water,there was no significant difference with them before anesthesia.This study suggested that the appropriate concentration of MS-222 in the transport process of C.gibelio is more than 60 mg/L,and the concentration in scientific researches is less than 100 mg/L.关键词
麻醉剂/MS-222/异育银鲫/氧化损伤Key words
Anesthetic/MS-222/Carassius auratus gibelio/Oxidative damage分类
农业科技引用本文复制引用
马之豪,袁健美,仇明,徐超楠,曹永军,吕庭莉,李强,王加美,乔帼..MS-222对异育银鲫(Carassius auratus gibelio)麻醉复苏效果及抗氧化酶活性的影响[J].安徽农业科学,2025,53(2):77-83,7.基金项目
国家自然科学基金项目(32071615) （32071615）
盐城渔业高质量发展课题(YCSCYJ2021020). （YCSCYJ2021020）
