安徽农业科学2025，Vol.53Issue(2)：117-123,7.DOI:10.3969/j.issn.0517-6611.2025.02.025
基于表型性状和ISSR分析的5个核桃品种亲缘关系鉴定
Identification of Genetic Relationships Among Five Walnut Varieties Based on Phenotypic Traits and ISSR Analysis
摘要
Abstract
[Objective]Select the walnut variety Juglans regia'Yanyuanzao'with the largest cultivation area and the largest production in Si-chuan Province,and explore the genetic relationship of Juglans regia'Yanyuanzao'.[Method]This study selected five walnut varieties,Jug-lans regia'Yanyuanzao',Juglans sigillata'Santai',Juglans sigillata'Yangbi',Juglans regia'Wen185'and Juglans regia'Xinxin2'as re-search materials.The single fruit weight,kernel weight,kernel yield,nut diameter,fruit shape index,seed shell thickness,and kernel composition(crude protein)were measured,and the diversity of phenotypic characters and kernel component of five walnut cultivars were studied by the ANOVA,correlation,clustering and principal component.The genetic diversity of five walnut variety including'Yanyuanzao'were investigated by ISSR molecular marker technology.[Result]The single fruit weight,kernel weight,kernel yield,lateral diameter,transverse diameter,lon-gitudinal diameter,fruit shape index,seed shell thickness,and kernel composition(crude fat and crude protein content)of the five walnut va-rieties showed extremely significant or significant differences(P＜0.01 or P＜0.05).The results of cluster and principal component analysis showed that the five walnut varieties could be clustered into three major classes,with the class Ⅰ being'Wen185',the class Ⅱ being'Xinxin2'and'Yanyuanzao'and'Yangbi',and the class Ⅲ was'Santai'.10 primers with clear bands,bright background,high reproduc-ibility,good polymorphism and high stability were screened by using ISSR molecular markers.The results of polymorphism analysis showed that the mean number of effective alleles of the five walnut varieties was 1.337 0,the mean gene diversity index was 0.193 5,and the mean Shannon information index was 0.285 8.The clustering and principal component analysis showed that'Yanyuanzao'and'Yangbi'were clustered to-gether,indicating that the relatives of them were closer,and'Wen185'and'Xinxin2'were clustered together in the same area,and the'San-tai'was clustered into one category.[Conclusion]Cluster analysis of the fruit phenotypic traits,kernel components and ISSR molecular markers of five walnut varieties showed that,'Yanyuanzao'and'Yangbi'had a close genetic relationship.关键词
核桃品种/表型性状/果仁成分/ISSR分子标记Key words
Walnut varieties/Phenotypic traits/Fruit kernel ingredients/ISSR molecular marker分类
农业科技引用本文复制引用
吴雨桦,李丕军,熊福博,李润,刘华,白斌,郑崇文,王静,冯春..基于表型性状和ISSR分析的5个核桃品种亲缘关系鉴定[J].安徽农业科学,2025,53(2):117-123,7.基金项目
四川省"十四五"育种攻关项目(2021YFYZ0032-07) （2021YFYZ0032-07）
四川省科技厅重点研发项目(2021YFN0005) （2021YFN0005）
四川省创新团队项目(2024LCTD0208). （2024LCTD0208）
