河北科技大学学报2025，Vol.46Issue(1)：100-107,8.DOI:10.7535/hbkd.2025yx01011
产CpxP蛋白枯草芽孢杆菌的发酵优化及中试生产
Fermentation optimization and pilot production of Bacillus subtilis ZY1 producing CpxP
摘要
Abstract
In order to obtain the optimal conditions for liquid fermentation of Bacillus subtilis ZY1 producing CpxP protein and prepare microecological bactericides,the fermentation process of Bacillus subtilis ZY1 was optimized by single factor test and response surface test.The results show that the optimal carbon source,nitrogen source and inorganic salt of fermentation medium are starch,soybean meal and NaCl,respectively.The optimal medium formulation is starch at 2.12 g/L,soybean meal at 5.90 g/L,and NaCl at 5.21 g/L,with a viable bacteria count of 9.63×108 CFU/mL,which is 11.04 times higher than that before the medium optimization.The optimum initial pH value,temperature,rotation speed and inoculation amount for shake flask fermentation are 7.0,37℃,200 r/min and 2%,respectively.The viable bacteria number is 1.05×109 CFU/mL,which is 9.0%higher than that of optimized medium.During the pilot production process of the microecological bactericides based on 5 t fermentation tank system with 10%inoculated quantity,the count of viable ZY1bacteria reaches 1.05×1011 CFU/mL,which is 100 times higher than that after shaker optimization.When the spore rate reaches 90%at the end of fermentation,1 012 kg bactericides are prepared by spray drying,and the count of viable bactericide is 8.5×109 CFU/g.The research results can provide basic materials for further research on the biocontrol function of CpxP protein in animal bacterial diarrhea disease.关键词
蛋白质工程/CpxP蛋白/枯草芽孢杆菌/发酵优化/响应面/中试生产Key words
protein engineering/CpxP protein/Bacillus subtilis/optimization of fermentation/response surface/pilot pro-duction分类
生物科学引用本文复制引用
董荣荣,张焕,于欣然,刘浩,周晓辉..产CpxP蛋白枯草芽孢杆菌的发酵优化及中试生产[J].河北科技大学学报,2025,46(1):100-107,8.基金项目
国家药典委员会科研项目(2021Y04) （2021Y04）
河北省自然科学基金生物医药联合基金重点项目(C2020208020) （C2020208020）
河北省农业科技成果转化资金项目(202460101030040) （202460101030040）
