东南园艺2024，Vol.12Issue(6)：445-454,10.DOI:10.20023/j.cnki.2095-5774.2024.06.001
猕猴桃根腐病菌的分离鉴定及不同种质的抗性分析
Isolation and Identification of the Pathogen Causing Kiwifruit Root Rot and the Resistance Analysis of Different Germplasms to the Pathogen
摘要
Abstract
[Objective]The type of pathogen causing the root rot of kiwifruits in Mingxi County,Sanming City,Fujian Province need to be clarified.And the differences in resistance to the pathogen among different kiwifruit germplasms also need to be studied.[Method]The pathogen was isolated by using tissue separation method.The morphological observation and molecular biological methods were used to identify the pathogen.Then,its pathogenicity was tested according to Koch's postulates.And the resistance to the pathogen among different kiwifruit germplasms was analyzed.[Result]A pathogen(named 0814-1)was isolated from typical root rot tissues of kiwifruits.The colony was white on the surface,and white or orange on the back,with an irregular margin.The ITS an TEF were cloned from 0814-1 and sequenced,and the blast analysis in NCBI database showed that they shared more than 99%identity with that of Fusarium concentricum.The phylogenetic analysis also revealed that 0814-1 clustered with F.concentricum in the same branch.Pathogenicity test results showed that the symptoms caused by 0814-1 were consistent with that in the field,fulfilling Koch's postulates.Based on the results of morphology,molecular biology,and Koch's postulates,the isolated pathogen was identified as F.concentricum.Resistance analysis showed that there were significant differences in resistance to the pathogen 0814-1 among those germplasms,and'Jinkui'and'White'were screened as disease-resistant germplasms.[Conclusion]A pathogen causing kiwifruit root rot was isolated and identified as F.concentricum,then two disease-resistant germplasms were screened.Those results laid the foundation for the integrated control of kiwifruit root rot and resistance breeding.关键词
猕猴桃/根腐病/轮纹镰孢菌/抗性分析Key words
Kiwifruit/Root rot/Fusarium concentricum/Resistance analysis分类
农业科技引用本文复制引用
冯新,刘晓驰,叶炜,路喻丹,陈桂信,高敏霞,赖瑞联,陈义挺..猕猴桃根腐病菌的分离鉴定及不同种质的抗性分析[J].东南园艺,2024,12(6):445-454,10.基金项目
中央引导地方科技发展专项(2023L3025) （2023L3025）
"十四五"福建省种业创新与产业化工程(zycxny2021010) （zycxny2021010）
福建省自然科学基金(2023J01367) （2023J01367）
福建省属公益类科研院所基本科研专项(2023R1087) （2023R1087）
福建省农业科学院科技创新团队建设(CXTD2021009-2) （CXTD2021009-2）
