东南园艺2024，Vol.12Issue(6)：455-463,9.DOI:10.20023/j.cnki.2095-5774.2024.06.002
山茶花花色苷等次生代谢产物积累特征与关键基因表达分析
Accumulation Characteristics and Key Gene Expression Analysis Involved in Anthocyanin Biosynthesis in Camellia japonica
摘要
Abstract
[Objective]This study aims to investigate the accumulation of metabolites and the expression characteristics of key genes in camellia(C.)japonica flowers with varying flower colors,as well as to analyze the relationship between the two factors.[Method]Six different color traits of C.japonica petals were selected as materials to determine the contents of anthocyanins,total phenols,flavonoids,and proanthocyanidins.Real-time fluorescence quantitative PCR was employed to assess the expression levels of genes associated with product synthesis,and a network diagram illustrating the relationship between metabolite accumulation and gene expression was constructed.[Result]The accumulation of anthocyanins in dark colored cultivars of C.japonica were found to be higher than that in light and white cultivars.Notably,the purple-red cultivar'Midnight'exhibited significantly higher contents of anthocyanins,flavonoids,and proanthocyanidins,reaching 572.18 µg/g,18.93 mg/g,and 97.29 mg/g,respectively.Furthermore,the total phenolic content in dark and light colored cultivars exceeded that of moderately colored cultivars,with the red cultivar'Chidan'displaying the highest content at 103.13 mg/g,while'Midnight'also showed a relative high level of accumulation.Quantitative expression analysis revealed that gene expression levels varied specifically among different cultivars.'Midnight'demonstrated high expression of upstream genes(CHS and F3H)and low expression of downstream genes(F3'H,DFR,ANS,and UFGT).Correlation analysis indicated that flavonoid and proanthocyanidin accumulation was positively correlated with the expression of F3H,ANS,and UFGT.Additionally,anthocyanin accumulation was positively correlated with the expression of CHS,CHI,and F3'H,and negatively correlated with F3H,DFR,and ANS.[Conclusion]The C.japonica'Midnight'exhibits a high accumulation of anthocyanins,total phenols,flavonoids,and proanthocyanidins.The upstream and downstream genes in the synthesis pathway have both promoting and inhibitory effects on anthocyanin synthesis and accumulation,respectively,while downstream genes generally promote the accumulation of flavonoids and proanthocyanidins.关键词
山茶花/花色苷/类黄酮/代谢产物/基因表达Key words
Camellia japonica/Anthocyanin/Flavonoid/Metabolite/Gene expression分类
农业科技引用本文复制引用
赖恭梯,潘若,贺丽媛,林俊璇,郭奥琳,车建美,赖呈纯..山茶花花色苷等次生代谢产物积累特征与关键基因表达分析[J].东南园艺,2024,12(6):455-463,9.基金项目
福建省属公益类科研院所基本科研专项(2021R10320013) （2021R10320013）
