实用临床医药杂志2024，Vol.28Issue(24)：82-87,6.DOI:10.7619/jcmp.20243912
血浆长链非编码RNA浆细胞瘤变异易位基因1、微小RNA-143-3p表达水平与类风湿关节炎患者疾病活动度及预后的相关性分析
Correlations of the expression levels of plasma long non-coding RNA plasmacytoma variant translocation 1 and microRNA-143-3p with disease activity and prognosis in patients with rheumatoid arthritis
摘要
Abstract
Objective To investigate the correlations of the expression levels of plasma long non-coding RNA plasmacytoma variant translocation 1(LncRNA PVT1)and microRNA-143-3p(miR-143-3p)with disease activity and prognosis in patients with rheumatoid arthritis(RA).Meth-ods A total of 129 RA patients admitted to our hospital from April 2021 to April 2023 were selected as disease group.According to the 28-joint disease activity scores(DAS28),the patients were divid-ed into stable disease group(n=28),mildly active group(n=42),moderately active group(n=35),and severely active group(n=24).According to the prognosis of the patients after 6 months of treatment,they were divided into good prognosis group(n=88)and poor prognosis group(n=41),and 110 healthy people who underwent physical examinations in our hospital during the same period were included as control group.The expression levels of plasma LncRNA PVT1 and miR-143-3p were detected using quantitative real-time fluorescent polymerase chain reaction(qRT-PCR).The targeting relationship between LncRNA PVT1 and miR-143-3p was predicted using the Target Scan Human website.Multivariate Logistic regression analysis was conducted to explore the factors influ-encing the prognosis of RA patients.Receiver operating characteristic(ROC)curve was performed to assess the predictive value of plasma LncRNA PVT1 and miR-143-3p for the prognosis of RA pa-tients.Results The plasma LncRNA PVT1 level in the disease group was higher than that in the control group,while the plasma miR-143-3p level was lower(P＜0.05).The plasma LncRNA PVT1 levels decreased sequentially in the severe activity,moderate activity,mild activity,and dis-ease stable groups,while the plasma miR-143-3p levels increased sequentially(P＜0.05).The proportion of patients positive for human leukocyte antigen-DR4(HLA-DR4),DAS28,and plasma LncRNA PVT1 expression level were higher in the poor prognosis group than in the good prognosis group,while the plasma miR-143-3p level was lower(P＜0.05).Plasma LncRNA PVT1,miR-143-3p expression,HLA-DR4 positivity,and DAS28 were factors influencing the prognosis of RA patients(P＜0.05).LncRNA PVT1 and miR-143-3p had targeted binding sites,and there was a negative correlation between them(P＜0.05).The area under the curve(AUC)for the combined prediction of plasma LncRNA PVT1 and miR-143-3p for prognosis of RA patients was 0.914,which was superior to the individual diagnosis of LncRNA PVT1 or miR-143-3p(Z=2.159,P=0.031;Z=2.108,P=0.035).The sensitivity and specificity of the combined diagnosis were 95.12％and 78.41％,respectively.Conclusion The plasma LncRNA PVT1 level gradually increases,while the plasma miR-143-3p level gradually decreases with increasing RA disease activity.The combined detection of the two biomarkers has high predictive value for the prognosis of RA patients.关键词
类风湿关节炎/长链非编码RNA浆细胞瘤变异易位基因1/微小RNA-143-3p/疾病活动度/预后Key words
rheumatoid arthritis/long non-coding RNA plasmacytoma variant translocation 1/microRNA-143-3p/disease activity/prognosis分类
医药卫生引用本文复制引用
崔欣,闫晓雯,孙晓,付妍妍,陶然..血浆长链非编码RNA浆细胞瘤变异易位基因1、微小RNA-143-3p表达水平与类风湿关节炎患者疾病活动度及预后的相关性分析[J].实用临床医药杂志,2024,28(24):82-87,6.基金项目
河北省2024年度医学科学研究课题计划项目(20240691) （20240691）
