昆明医科大学学报2025，Vol.46Issue(1)：36-42,7.DOI:10.12259/j.issn.2095-610X.S20250105
高良姜素通过影响Hippo/YAP通路抑制宫颈癌Hela细胞迁移和侵袭
Galangin Inhibits the Migration and Invasion of Cervical Cancer Hela Cells Through Hippo/YAP Pathway
摘要
Abstract
Objective To investigate the effects of galangin on the migration and invasion abilities of cervical cancer Hela cells and its potential mechanisms.Methods Hela cells were treated with different concentrations of galangin(0,5,10,20,40,60,80,100 µmol/L)for 48 hours,and CCK-8 assay was used to assess the impact of galangin on cell viability and to determine the half-maximal lethal concentration(IC50)of galangin.Hela cells were divided into a control group(0 μmol/L)and a galangin group(40 μmol/L treatment).Scratch wound healing assays and Transwell chamber assays were conducted to evaluate the migration and invasion abilities of the cells in each group.Western Blot was used to detect the protein expression of E-cadherin and N-cadherin.DIA quantitative proteomics technology was used to detect and screen the differentially expressed proteins between the two groups.Biological function enrichment analysis of the differential genes was performed using the KEGG Pathway and Gene Set Enrichment Analysis(GSEA)methods.Western Blot was used to verify the expression levels of Hippo/YAP signaling pathway-related proteins YAP and p-YAP.Results Compared to the control group,galangin(40 μmol/L)significantly inhibited the viability of Hela cells in a concentration-dependent manner(P<0.001).Compared with the control group,the scratch healing ability and invasion ability of cervical cancer Hela cells treated with galangin(40 μmol/L)were significantly reduced(P<0.001).The expression of E-cadherin protein was increased(P<0.05)and the expression of N-cadherin protein was decreased(P<0.001)in the galangin group(40 μmol/L)compared to the control group.KEGG and GSEA enrichment results indicated that the inhibition of malignant progression in cervical cancer by galangin was significantly associated with the Hippo/YAP signaling pathway.Western Blot confirmed that the expression level of the hallmark protein p-YAP in the Hippo signaling pathway was increased(P<0.01),while the expression level of YAP protein was decreased(P<0.05).Conclusion Galangin inhibits the proliferation,migration and invasion abilities of Hela cells in a dose-dependent manner.The underlying mechanism might be associated with the activation of the Hippo/YAP signaling pathway.关键词
高良姜素/宫颈癌/迁移/侵袭/Hippo/YAP信号通路Key words
Galangin/Cervical cancer/Migration/Invasion/Hippo/YAP signaling pathway分类
医药卫生引用本文复制引用
严怡然,沈成万,尚香玉,冯婵,李金秋,阿仙姑·哈斯木..高良姜素通过影响Hippo/YAP通路抑制宫颈癌Hela细胞迁移和侵袭[J].昆明医科大学学报,2025,46(1):36-42,7.基金项目
福建省自然科学基金(2021J011174) （2021J011174）
宁德师范学院科技项目(2021Q105) （2021Q105）
