湖南农业大学学报（自然科学版）2024，Vol.50Issue(6)：31-35,93,6.DOI:10.13331/j.cnki.jhau.2024.06.004
一株米根霉的鉴定及其发酵产乳酸研究
Identification of a strain of Rhizopus oryzae and study on its fermentation production of lactic acid
摘要
Abstract
Rhizopus oryzae produces important organic acids such as lactic acid,and has important application value in many fields.In this study,a fungus was isolated from Baijiu Daqu of Beidacang,Heilongjiang Province,and the strain was identified and named as Rhizopus oryzae WSW-LYN-001 using internal transcribed spacer(ITS)identification.The results showed that using glucose as the carbon source,after 48 hours,the pH of fermentation broth reached around 3.2.After 84 hours of fermentation,the lactate production maintained at around 70 g/L.The passage number could affect the morphology of fungal hyphae(flocculent and globular mycelium clusters appeared),which in turn affected lactate production.After 60 hours of fermentation,the lactate production in the fermentation broth of spherical mycelium was higher than that of flocculent mycelium.After pH optimization,the spherical mycelium produced the maximum lactic acid yield of 134.25 g/L after 60 hours of fermentation at an initial pH of 7.关键词
米根霉/rDNA/内转录间隔区(ITS)/菌种鉴定/乳酸生产Key words
Rhizopus oryzae/rDNA/internal transcribed spacer(ITS)/strains identification/lactic acid production分类
生物科学引用本文复制引用
王世伟,王卿惠,刘伊娜..一株米根霉的鉴定及其发酵产乳酸研究[J].湖南农业大学学报（自然科学版）,2024,50(6):31-35,93,6.基金项目
黑龙江省教育厅基本科研业务费科研项目(1353309482) （1353309482）
2021年度黑龙江省大学生创新创业训练计划项目(202110232101) （202110232101）
