湖南农业大学学报（自然科学版）2024，Vol.50Issue(6)：36-42,7.DOI:10.13331/j.cnki.jhau.2024.06.005
基于非靶向代谢组学分析阿维菌素发酵液的挥发性代谢物
Analysis of volatile metabolites in avermectin fermentation broth based on untargeted metabolomics
摘要
Abstract
This experiment aimed to investigate the components of volatile substances in avermectin fermentation broth and the volatile differential metabolites across different fermentation periods.Utilizing headspace solid-phase microextraction(HS-SPME)and gas chromatography-mass spectrometry(GC/MS)non-targeted metabolomics technology in conjunction with odor activity value(OAV),the volatile components in 36 h and 168 h fermentation broths were qualitatively and quantitatively analyzed.The differential volatile metabolites were analyzed by principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA)and cluster analysis(HCA).The findings revealed that 396 volatile organic compounds were detected in the fermentation broth of avermectin,primarily consisted of hydrocarbons,halogenated hydrocarbons,alcohols,ketones,esters,heterocyclic compounds and terpenoids;among them,there were 11 substances with OAV>200,which were the key components contributing to the odor of avermectin fermentation broth.There were two distinct metabolites identified from the two fermentation periods,namely 5-methyl-2-heptanone and o-cymene.关键词
阿维菌素发酵液/顶空固相微萃取/气相色谱-质谱/挥发性有机化合物/异味活度值Key words
avermectin fermentation broth/headspace solid-phase microextraction(HS-SPME)/gas chromatography-mass spectrometry(GC/MS)/volatile organic compounds(VOC)/odor activity value(OAV)分类
化学化工引用本文复制引用
张琪,岳思君,武彦路,焦焱辉..基于非靶向代谢组学分析阿维菌素发酵液的挥发性代谢物[J].湖南农业大学学报（自然科学版）,2024,50(6):36-42,7.基金项目
宁夏回族自治区重点研发计划项目(2021BEG02003) （2021BEG02003）
宁夏大学新华学院科学研究基金项目(23XHKY04) （23XHKY04）
