湖南农业大学学报（自然科学版）2024，Vol.50Issue(6)：43-53,11.DOI:10.13331/j.cnki.jhau.2024.06.006
产黑芥子酶乳酸菌的筛选与鉴定及产酶条件的优化
Screening and identification of myrosinase-producing lactic acid bacteria and optimization of enzyme production conditions
摘要
Abstract
In order to screen myrosinase-producing lactic acid bacteria,palladium chloride assay was used to determine the myrosinase enzyme activity,and the myrosinase-producing strains were screened from 39 strains of plant-derived lactic acid bacteria.The screened strains were identified by morphological characteristics,physiological and biochemical experiments in combination with 16S rDNA sequence analysis,and the enzyme-producing conditions of the strains were optimized by using the single-factor experiment and response surface method.The results showed that three strains of lactic acid bacteria(named as B244,161 and 602,respectively)could effectively degrade glucosinolates in the growth environment.Strain B244 was identified as Pediococcus pentosaceus,and 161 and 602 were both identified as Limosilactobacillus fermentum.Pediococcus pentosaceus had a strong ability to produce myrosinase,and the optimal culture conditions for the myrosinase production were as follows:4.2 mmol/L glucosinolates was added to LB medium,the mass concentration of peptones was adjusted to 14.3 g/L with the initial pH of 6.9 and incubation time of 16 h at 40℃.The myrosinase activity of B244 under the optimized condition reached 117.2 U/L,which was 52.9%higher than that(76.65 U/L)before optimization.关键词
乳酸菌/黑芥子酶/硫代葡萄糖苷/产酶条件优化/响应面法Key words
lactic acid bacteria/myrosinase/glucosinolates/optimization of enzyme producing/response surface method分类
轻工纺织引用本文复制引用
杨嘉琪,赵玲艳,邓雪盈,邓放明..产黑芥子酶乳酸菌的筛选与鉴定及产酶条件的优化[J].湖南农业大学学报（自然科学版）,2024,50(6):43-53,11.基金项目
湖南省自然科学基金面上项目(2021JJ30315) （2021JJ30315）
国家现代农业特色蔬菜产业技术体系建设专项(CARS-24-E-02) （CARS-24-E-02）
长沙市 2023年知识产权公共项目(长知发[2023]33号-3-3) （长知发[2023]33号-3-3）
