湖南农业大学学报（自然科学版）2024，Vol.50Issue(6)：62-70,9.DOI:10.13331/j.cnki.jhau.2024.06.008
猕猴桃突变体库的构建及表型变异分析
Construction of a kiwifruit mutant library and analysis of phenotypic variations
摘要
Abstract
To accelerate the development of functional genomic research,the mutant library of'Hongyang'kiwifruit was constructed.The callus of'Hongyang'kiwifruit were treated with 60Co-γ rays at 10 distinct radiation doses:10,25,50,75,100,150,200,300,400,and 500 Gy,with 0 Gy serving as the control,and the phenotypic variation characteristics of the mutagenic progeny were analyzed.The results indicated that after mutagenic treatment with a dose of 100 Gy,the mutation rate of callus tissue from'Hongyang'kiwifruit increased to 35.9%,while the differentiation rate reached 77.8%.Based on these findings,a radiation dose of 100 Gy was selected as the further studied condition.After treating 1 680'Hongyang'kiwifruit callus under optimal mutagenesis conditions,a kiwifruit mutant library contained 1 026 regenerated seedlings of'Hongyang'kiwifruit was constructed.The mutant library had rich variation in leaf,plant type and other characters,with a total variation frequency of 35.9%.关键词
猕猴桃/60Co-γ/突变体库/表型分析Key words
kiwifruit/60Co-γ/mutant library/phenotypic analysis分类
农业科技引用本文复制引用
苑平,杨莉颖,汤佳乐,徐海,何科佳,沈婉琪,杨博智,卜范文..猕猴桃突变体库的构建及表型变异分析[J].湖南农业大学学报（自然科学版）,2024,50(6):62-70,9.基金项目
长沙市自然科学基金项目(KQ2202351) （KQ2202351）
湖南省农业科技创新资金项目(2024CX66) （2024CX66）
国家柑橘产业技术体系猕猴桃长沙综合试验站(CARS-26) （CARS-26）
湖南省现代农业(水果)产业技术体系猕猴桃育种与栽培岗位专家(HARS-09) （水果）
