北方农业学报2024，Vol.52Issue(5)：21-28,8.DOI:10.12190/j.issn.2096-1197.2024.05.03
基于ISSR的8个羊肚菌菌株遗传多样性分析
Genetic diversity analysis of 8 Morchella esculents strains based on ISSR
摘要
Abstract
[Objective]To clarify the phylogenetic relationship and diversity of Morchella esculents germplasm resources,and to provide reference for the selection and breeding of new varieties adapted to the cultivation in the western region of Inner Mongolia.[Methods]8 Morchella esculents strains(A3,Zhao'erliu,Yangguifei,Jinqiu,DY008,A Lu,001 and Neishilin)were used as experimental materials,ISSR molecular marker technology was used to perform cluster analysis and genetic diversity comparison on 8 strains of Morchella esculents.[Results]Eight Morchella esculents strains were detected by PCR using 100 ISSR primers,12 ISSR primers were selected,and 76 DNA bands were amplified.There were 59 effective bands,and the polymorphism ratio was 75.83％.The gene diversity index(H)was 0.206 6,and Shannon's information index(I)was 0.330 0.The maximal variation of genetic distance was 1.072 6.When the GS value was about 0.38,the strains were divided into 5 groups.[Conclusion]There was significant genetic variability among the tested Morchella esculents strains.关键词
羊肚菌/ISSR/遗传多样性/聚类分析Key words
Morchella esculents/ISSR/Genetic diversity/Cluster analysis分类
农业科技引用本文复制引用
于海宾,鲍红春,李小雷,任益芳,宗海龙,庞杰,于传宗,王燕春,张文光..基于ISSR的8个羊肚菌菌株遗传多样性分析[J].北方农业学报,2024,52(5):21-28,8.基金项目
内蒙古自治区科技计划项目(2020GG0223,2021GG0175,2022YFHH0110) （2020GG0223,2021GG0175,2022YFHH0110）
内蒙古自治区"揭榜挂帅"项目(2022JBGS0028) （2022JBGS0028）
