北方农业学报2024，Vol.52Issue(5)：29-37,9.DOI:10.12190/j.issn.2096-1197.2024.05.04
利用分子标记检测59份番茄种质对3种病毒的抗性研究
Detection of resistance to three viruses in 59 tomato germplasms by molecular markers
摘要
Abstract
[Objective]To screen the tomato germplasm resistance to Tomato masaic virus(ToMV),Tomato spotted wilt virus(TSWV)and Tomato yellow leaf curl virus(TYLCV),and lay a foundation for tomato antiviral breeding in Inner Mongolia Autonomous Region.[Methods]Using six-leaf stage tomato leaves as experimental materials,59 tomato germplasms were analyzed for their resistance to ToMV,TSWV,and TYLCV,and DNA extraction and molecular marker detection were performed.[Results]Nine varieties demonstrated resistance to TYLCV,including 1 cherry tomato,1 strawberry tomato,and 7 fresh tomatoes.Five varieties demonstrated resistance to TSWV,including 3 cherry tomatoes and 2 strawberry tomatoes.Fourteen varieties demonstrated resistance to ToMV,including 3 strawberry tomatoes and 11 fresh tomatoes.[Conclusion]In the 59 tomato germplasms,fresh tomatoes resistant to viruses accounted for 30.51%,strawberry tomatoes accounted for 10.17%,and cherry tomatoes accounted for 6.78%;There were 14 ToMV-resistant germplasms,5 TSWV-resistant germplasms,and 9 TYLCV-resistant germplasms,so 47.46%of the germplasms contained disease resistance genes.关键词
分子标记检测/抗性基因/番茄花叶病毒/番茄斑萎病毒/番茄黄化曲叶病毒Key words
Molecular maker detection/Resistance genes/Tomato mosaic virus/Tomato spotted wilt virus/Tomato yellow leaf curl virus分类
农业科技引用本文复制引用
孟彻,高婧,杨永青,付崇毅,廉勇,李正男,刘燕,张磊..利用分子标记检测59份番茄种质对3种病毒的抗性研究[J].北方农业学报,2024,52(5):29-37,9.基金项目
内蒙古自治区"揭榜挂帅"项目(2022JBGS0028) （2022JBGS0028）
内蒙古自治区自然科学基金项目(2023LHMS03020,2022QN03018) （2023LHMS03020,2022QN03018）
内蒙古自治区本级引进人才科研支持项目(2022) （2022）
