北方农业学报2024，Vol.52Issue(5)：59-70,12.DOI:10.12190/j.issn.2096-1197.2024.05.07
鹰嘴豆黄酮类化合物反相高效液相色谱法的建立及其体外药效评价
Establishment of reversed phase high performance liquid chromatography for flavonoids in chickpeas and evaluation of their in vitro efficacy
摘要
Abstract
[Objective]To establish a reversed phase high performance liquid chromatography method for the effective components of chickpea flavonoids,and evaluate the in vitro pharmacological effects of chickpeas and chickpea total flavonoids through antibacterial tests.[Methods]Reversed phase high performance liquid chromatography was used for analysis,Zorbax SB-C18(4.6 mm×150.0 mm,5 μm)column was used,with mobile phase A(methanol)∶B(0.5%phosphoric acid aqueous solution)=45∶55.The flow rate was 0.8 mL/min for equal elution,and the column temperature was 30℃.The precision,stability,repeatability and sample recovery were verified by HPLC.Filter paper diffusion method,Oxford cup method and micro broth dilution method were used to investigate the antibacterial effect of chickpea(100 mg/mL)and chickpea total flavonoids(20 mg/mL).[Results]After equal elution,three flavonoids,rutin,naringin dihydrochalcone,and quercetin were well separated,and their chromatographic peaks showed good separation within 30 min,with a linear range of 0.003 75-0.060 00 mg/mL(R2=0.996-0.999).The stability and repeatability test results indicate that,the content of rutin was 0.032 mg/g,and the content of naringin dihydrochalcone was 0.052 mg/g,quercetin was not detected.In the antibacterial effect experiment,the filter paper diffusion method and Oxford cup method did not appear bacteriostatic circle,and the pore plates of the micro broth dilution method bacteriostatic experiment were muddy.[Conclusion]The reversed phase high performance liquid chromatography method used in this experiment has certain reliability and is suitable for the separation and determination of two flavonoids,rutin and naringin dihydrochalcone in chickpeas.Through the verification of three antibacterial methods,chickpea and chickpea total flavonoids did not have obvious antibacterial effect.关键词
鹰嘴豆/总黄酮/反相高效液相色谱法/抑菌效果Key words
Chickpeas/Total flavonoids/Reversed phase high performance liquid chromatography/Antibacterial effect分类
化学化工引用本文复制引用
海婷玉,卫丁一,丁昱,熊青山,何子涵,戴小华,吴星霖..鹰嘴豆黄酮类化合物反相高效液相色谱法的建立及其体外药效评价[J].北方农业学报,2024,52(5):59-70,12.基金项目
国家自然科学基金项目(31960718) （31960718）
2021年新疆农业大学研究生科研创新项目(XJAUGRI2021032) （XJAUGRI2021032）
