北方农业学报2024，Vol.52Issue(5)：71-83,13.DOI:10.12190/j.issn.2096-1197.2024.05.08
不同来源淫羊藿的质量评价
Quality evaluation of Epimedii Folium from different sources
摘要
Abstract
[Objective]To constructed the quality evaluation methods of Epimedii Folium from different sources.[Methods]High performance liquid chromatography(HPLC)was used to determine the four flavonoids of epimedin A,epimedin B,epimedin C and icariin of the 30 batches of Epimedii Folium samples.The content of total flavonoids in the samples were determined by ultraviolet spectrophotometer(UV),and the alcohol soluble extract and total ash were also measured.Using grey relational analysis(GRA)combined with SPSS 27.0 and Simca 14.1 software for principal component analysis and cluster analysis,evaluate the quality of Epimedii Folium from different sources.[Results]The optimal reference sequence correlation,the worst reference sequence correlation and the relative correlation of the 30 batches of Epimedii Folium samples were 0.26-0.62,0.35-0.76 and 0.27-0.63,respectively.The results of principal component analysis show that the eigenvalues of the three principal components with eigenvalues greater than 1 were 3.179,1.964 and 1.185,respectively.The variance contribution rates were 39.733%,24.554%and 14.818%,respectively.The cumulative variance interpretation rate was 79.102%.In the cluster analysis,when the square Euclidean distance was 5,the samples were divided into 6 categories.[Conclusion]The GRA method combined with SPSS 27.0 and Simca 14.1 can evaluate the quality of Epimedii Folium comprehensively and reliably.关键词
淫羊藿/灰色关联度分析法/主成分分析/聚类分析/质量评价Key words
Epimedii Folium/Grey relational analysis/Principal component analysis/Cluster analysis/Quality evaluation分类
医药卫生引用本文复制引用
何洁,黄文,杨洁,王仕宝,丁伟,崔艳丽,张萌萌,黄东萍,张慧,何志鹏..不同来源淫羊藿的质量评价[J].北方农业学报,2024,52(5):71-83,13.基金项目
2017年中医药公共卫生服务补助专项项目(财社[2017]66号) （财社[2017]66号）
2023年汉中职业技术学院科研课题(2023YJN20,2023YJN21) （2023YJN20,2023YJN21）
2019年汉中青年科技创新团队(汉市科发[2019]26号) （汉市科发[2019]26号）
2023年度汉中职业技术学院数学建模青年科研创新团队(汉职院发[2023]85号) （汉职院发[2023]85号）
