农业环境科学学报2024，Vol.43Issue(12)：2959-2973,15.DOI:10.11654/jaes.2023-1067
连续配施有机肥对茶园土壤细菌群落结构及功能类群的影响
Effects of continuous chemical fertilizer combined with organic manure on soil bacterial community structure and functional groups in tea gardens
摘要
Abstract
To evaluate the effects of combined application of organic and chemical fertilizers on the structure and diversity of soil bacterial communities,we conducted a six-year field plot experiment on a tea garden in acid red soils in the subtropical zones of hilly areas of eastern Fujian.The five treatments included no fertilizer(CK),100% chemical fertilizers(CF),100% chemical fertilizer+clean-cultivated(CFC),organic manure combined with chemical fertilizer(OF),and organic manure combined with chemical fertilizer+green manure(OFM).The effects of different fertilization practices on bacterial community diversity,community structure,functional groups,and co-occurrence network structure in acid tea garden soils were analyzed with Illumina Novaseq PE 300 high-throughput sequencing technology.The results showed that:compared with CK treatment,CF treatment decreased soil pH,but significantly increased soil alkaline nitrogen and total phosphorus.Compared with CK treatment,OF and OFM treatments increased available nutrients and soil organic matter content while increasing soil pH.Compared with CK treatment,CF and CFC treatments decreased the alpha diversity index of the soil bacterial community,while Sobs,Ace,Chao1,and Shannon indices(P<0.05).Non-metric Multidimensional Scaling and analysis of similarities analysis both showed a significant difference in soil bacterial community structure between the soils under different fertilization measures(R2=0.442 1,P=0.001),such that OF treatment enriched species that were beneficial to soil carbon and nitrogen cycling and inhibited pathogens.FAPROTAX function prediction showed that the chemoheterotrophy,aerobic chemoheterotrophy,nitrogen fixation,cellulolysis,and nitrate reduction was mainly an ecological function of tea garden soil bacteria.Compared with CK treatment,the abundance of functional groups of predatory or exoparasitic,photoheterotrophy,phototrophy,anoxygenic photoautotrophy S oxidizing,anoxygenic photoautotrophy,and nitrogen-cycling functional genes were improved in OF treatment.The treatments of fertilization increased the numbers of network edges,average connective degree,and average number of neighbors in the soil bacterial co-occurrence networks,indicating that the soil bacterial co-occurrence network was complex under all fertilizing treatments.The bacterial co-occurrence networks were dominated by positive interactions(58.03%-77.78% ),and the competition between species was strengthened after the implementation of the different fertilization measures.The fertilization measures increased the average path length and reduced the average clustering coefficient.This means that the response rate of bacterial action was slowed and the stability of the community structure was improved.Additionally,the stability of bacterial community structure treated by organic fertilizer combined with chemical fertilizer was better than that treated by chemical fertilizer alone.The results of the redundancy analysis and Mantel test showed that available phosphorus and pH were the main factors affecting the abundance and diversity of the soil bacterial community.In conclusion,organic manure combined with chemical fertilizer changes the community structure and the functional groups of soil bacteria,promotes soil bacteria carbon and nitrogen cycle metabolism,enriches beneficial bacteria,and increases the bacterial co-occurrence network scale,interaction,and stability,and thus is beneficial for building a healthy and stable bacterial community.关键词
茶园土壤/有机肥配施化肥/细菌多样性/细菌群落结构/细菌功能类群Key words
tea garden soil/organic manure combined with chemical fertilizer/bacterial diversity/soil bacterial community structure/bacterial functional group分类
农业科技引用本文复制引用
颜明娟,陈玉真,林诚,吴一群,郑向丽,王峰..连续配施有机肥对茶园土壤细菌群落结构及功能类群的影响[J].农业环境科学学报,2024,43(12):2959-2973,15.基金项目
福建省科技厅公益类科研专项(2021R1025004,2021R1029002) （2021R1025004,2021R1029002）
福建省自然科学基金项目(2021J01489) （2021J01489）
福建省农业科学院科技创新团队项目(CXTD2021006-1) （CXTD2021006-1）
农业科技创新联盟专项(CXLM202202)Special Public Welfare Research Program of Fujian Provincial Science and Technology Department(2021R1025004,2021R1029002) （CXLM202202）
Natural Science Foundation of Fujian Province,China(2021J01489) （2021J01489）
Scientific and Technological Innovation Team Construction Project of Fujian Academy of Agricultural Sciences(CXTD2021006-1) （CXTD2021006-1）
Special Program of Agricultural Science and Technology Innovation Alliance(CXLM202202) （CXLM202202）
