水产科技情报2025，Vol.52Issue(1)：42-48,7.DOI:10.16446/j.fsti.20231000111
饲料中添加枯草芽孢杆菌对赤点石斑鱼生长、免疫和肠道菌群的影响
Effects of dietary supplementation of Bacillus subtilis on growth,immunity and intestinal microbiota of red spotted grouper Epinephelus akaara
摘要
Abstract
To investigate the effects of dietary Bacillus subtilis on the growth,immunity,and intestinal microbiota of red spotted grouper(Epinephelus akaara),three diets were formulated to contain 0,0.5%,and 1.0%of B.subtilis to carry the feeding trial for 56 days.Each group had three replicates,and the fish had an average initial body weight of(92.13±7.91)g.The results showed that supplementation with B.subtilis in the feed had no sig-nificant effect on specific growth rate and feed coefficient of E.akaara(P>0.05).The supplementation with B.subtilis significantly increased the activities of serum superoxide dismutase(SOD),acid phosphatase(ACP),and alkaline phosphatase(AKP)(P<0.05),but had no significant effect on the activity of serum lysozyme(LZM)(P>0.05).The diet adding 1.0%B.subtilis significantly increased the total cholesterol(TG),high-density lipo-protein(HDL-C),and low-density lipoprotein(LDL-C)contents in the serum(P<0.05).The diet supplemented with B.subtilis increased the abundance of Clostridium sensu stricto,Turicibacter,and Bacillus,while reducing the abundance of Vibrio,Cetobacterium,and Erysipelothrix.In summary,the results indicate that adding B.subti-lis to the feed can enhance the immunity of red spotted grouper and improve the structure of their intestinal microbiota.关键词
枯草芽孢杆菌/赤点石斑鱼/免疫力/肠道菌群Key words
Epinephelus akaara/Bacillus subtilis/immunity/intestinal microbiota引用本文复制引用
马建忠,任鹏,陈舜,闫茂仓,陈万东,谢尚微,邵鑫斌,李安,张立宁..饲料中添加枯草芽孢杆菌对赤点石斑鱼生长、免疫和肠道菌群的影响[J].水产科技情报,2025,52(1):42-48,7.基金项目
温州市科研项目(KN29210009) （KN29210009）
南麂列岛国家级海洋自然保护研究项目(NJKJ-2021-002). （NJKJ-2021-002）
