山西农业科学2025，Vol.53Issue(1)：35-47,13.DOI:10.3969/j.issn.1002-2481.2025.01.04
白蜡树FcF6′H1基因的密码子偏好性分析
Codon Bias Analysis of FcF6′H1 Gene in Fraxinus chinensis
摘要
Abstract
In this study,the codon bias analysis of FcF6'H1 gene in Fraxinus chinensis was analyzed using CodonW software and EMBOSS online program,cluster analysis,neutral plot,ENC-plot and PR2-plot of the codon of FcF6'H1 gene in Rutaceae,Leguminosae,Umbelliferae,Oleaceae,Arabidopsis,tobacco and tomato was conduceted,the factors affecting information of the codon bias of the FcF6'H1 gene in Fraxinus chinensis were studied,the best receptors were obtained by comparing the codon usage frequencies of FcF6'H1 and model organisms.The rsults showed that the content of GC、GC1、GC2、GC3,and GC12 of FcF6'H1 in Fraxinus chinensis was 0.431 2,0.512 5,0.349 0,0.432 1,and 0.430 8,respectively,CAI was 0.216,and ENC was 57.84,indicating weak codon bias of FcF6'H1 in Fraxinus chinensis.The results of neutral analysis,ENC-plot analysis,and PR2-plot analysis indicated that codon bias of FcF6'H1 was affected by base mutation and natural selection.The results of CDS evolutionary tree and RSCU cluster analysis were not completely consistent,but both indicated that FcF6'H1 in Fraxinus chinensis and XM_023028611.1,XM_023036788.1,and XM_023034893.1 of Olea europaea var.sylvestris were aggregated into one category.Six optimal codons of F6'H1 gene were identified,they were CUC,AUC,AAG,GAG、UCG,and ACA.The codon usage frequence analysis showed that in the model plants,tomato tobacco were suitable for the genetic transformation receptors,and the Escherichia coli expression system was suitable for the heterologous expression vector of FcF6'H1.This study preliminarily clarified the codon usage pattern of FcF6'H1 gene in Fraxinus chinensis.关键词
白蜡树/FcF6'H1/密码子偏好性/ENC-plot/PR2-plot/异源表达Key words
Fraxinus chinensis/FcF6'H1/codon bias/ENC-plot/PR2-plot/heterologous expression分类
农业科技引用本文复制引用
孙晓春,黄文静,李会容..白蜡树FcF6′H1基因的密码子偏好性分析[J].山西农业科学,2025,53(1):35-47,13.基金项目
陕西省教育厅青年创新团队建设科研计划项目(21JP031) （21JP031）
陕西高校青年创新团队项目(陕教[2019]90号) （陕教[2019]90号）
陕西中医药大学秦药特色资源研究开发重点实验室开放课题(KF202326) （KF202326）
陕西中医药大学校级科研课题(2023GP32) （2023GP32）
云南省科技厅基础研究专项(202101AU070005) （202101AU070005）
