摘要
Abstract
Objective To investigate the impact of catalpol on intestinal inflammation in rats with severe acute pancreati-tis(SAP)by regulating the high mobility group protein Bl(HMGB1)-receptor for advanced glycation end products(RAGE)sig-naling pathway.Methods 30 SD rats were randomly selected,with 6 rats serving as sham group,and the remaining 24 rats were injected with sodium taurocholate solution to establish an SAP rat model.SAP rats were randomly grouped into SAP group,catalpol group(10 mg/kg catalpol),dexmedetomidine(DEX)group(2.5 μg/kg HMGB1-RAGE pathway activator DEX),and catalpol+DEX group(10 mg/kg catalpol+2.5 μg/kg DEX),with 6 rats in each group.SAP group and sham group were given equal a-mounts of physiological saline once a day,administered at 6 h and 30 h postoperatively,respectively.Rats arriveded at the endpoint of the experiment after 48 h postoperatively.ELISA method was applied to detect serum endotoxin,diamine oxidase(DAO),D-lactate,and intestinal tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).HE staining was applied to detect pathological changes in the ileum tissue.Western Blot was applied to detect intestinal barrier related proteins and HMGB1-RAGE pathway relat-ed proteins.Results The ileal mucosa and lamina propria of rats in sham group were normal and intact.Compared with sham group,intestinal mucosa of rats in SAP group was extensively damaged,accompanied by shorter and atrophied villi,gut score,endo-toxin,D-lactate,DAO,amylase,IL-6,TNF-α,HMGBl,and RAGE proteins were obviously increased(P＜0.05).ZO-1 and occlu-din proteins were obviously decreased(P＜0.05).Compared with SAP group,the villi of the ileum mucosa in the catalpol group recovered,with only slight edema and improved mucosal integrity,gut score,endotoxin,D-lactate,DAO,amylase,IL-6,TNF-α,HMGB1,and RAGE proteins were obviously decreased(P＜0.05).ZO-1 and occludin proteins were obviously increased(P＜0.05).The above indicators in DEX group showed opposite trends to those in catalpol group.DEX reversed the therapeutic effect of catalpol on intestinal inflammation in SAP rats.Conclusion Catalpol may have a therapeutic effect on intestinal inflammation in SAP rats by down-regulating the HMGB1-RAGE signaling pathway.关键词
梓醇/HMGB1-RAGE信号通路/重症急性胰腺炎/肠道炎症Key words
catalpol/HMGB1-RAGE signaling pathway/severe acute pancreatitis/intestinal inflammation分类
医药卫生
