实用医学杂志2025，Vol.41Issue(1)：7-14,8.DOI:10.3969/j.issn.1006-5725.2025.01.002
碱性神经酰胺酶1在硫酸钠葡聚糖诱导小鼠溃疡性结肠炎中的保护作用
Protective effect of basic alkaline ceramidase 1 in ulcerative colitis
摘要
Abstract
Objective To study the role and influence of basic Alkaline ceramidase 1 on mucosal barrier and immune regulation in ulcerative colitis(UC).Methods Acer1 knockout mice(Acer1 KO)were constructed,and the UC model was induced by continuous drinking water of sodium dextran sulfate(DSS)for 7 days,and the severity of symptoms of UC disease in C57 and Acer1 KO mice was compared.The expression levels of intestinal mucosal barriers(ZO-1,Occludin,Claudin,JAMA)were detected by RT-PCR and immunohistochemistry.Systemic immune response levels(IL-1b、IL-6、IL-23、IL-17、IL-10,and TNF-a)were assessed by ELISA and intestinal inflammatory infiltration levels(TNF-a、IL-1b、IL-6、IL-17、IL-21,etc.)were assessed by RT-PCR.Results There was no significant difference between C57 and Acer1 KO mice in free drinking water.In the DSS induced UC model,compared with C57 mice,the survival rate of Acer1 KO mice decreased,the weight decreased significantly,the mechanical barrier and mucus barrier protein expression levels decreased significantly,the intes-tinal epithelial barrier was seriously damaged,the inflammatory response was strong,and the cytokine infiltration was obvious,with statistical differences(P<0.05).Conclusion Acer1 can inhibit inflammatory infiltration by maintaining the integrity of intestinal mucosal barrier,preventing endotoxin,and delaying the progression of UC.关键词
碱性神经酰胺酶1/溃疡性结肠炎/机械屏障/黏液屏障/炎症损伤Key words
alkaline ceramidase 1/ulcerative colitis/mechanical barrier/mucus barrier/inflam-matory injury分类
医药卫生引用本文复制引用
何榕茂,方泽扬,张芸芸,吴友谅,梁世秀,王斯琪..碱性神经酰胺酶1在硫酸钠葡聚糖诱导小鼠溃疡性结肠炎中的保护作用[J].实用医学杂志,2025,41(1):7-14,8.基金项目
国家自然科学青年基金项目(编号:82300032) （编号:82300032）
广东省基础与应用基础研究基金省市联合基金项目(编号:2023A1515110161) （编号:2023A1515110161）
广州市科学技术局基础研究计划2024年度基础与应用基础研究专题项目(编号:2024A04J3537) （编号:2024A04J3537）
广州市科技局2024年度基础研究计划市校(院)联合资助"登峰医院"项目(编号:2024A03J1145) （院）
广州医科大学2023年科研能力提升计划 （）
