西北农业学报2025，Vol.34Issue(1)：169-176,8.DOI:10.7606/j.issn.1004-1389.2025.01.019
山西省蔬菜Botrytis cinerea对啶酰菌胺的抗性检测及生物学性状
Resistance Detection of Botrytis cinerea to Boscalid and Biological Traits in Shanxi Province
摘要
Abstract
To elucidate the resistance of Botrytis cinerea to boscalid in protected vegetable of Shanxi province,the resistance levels of 312 B.cinerea isolates to boscalid were determined by the mycelium growth rate method.The mutation sites in the SdhB gene and biological fitness,and the cross-resist-ance between boscalid and the other four fungicides(pyraclostrobin,difenoconazole,pyrimethanil and prochloraz)were investigated in both boscalid-resistant and sensitive isolates.The results showed that B.cinerea isolates exhibited resistance to boscalid(resistance frequency of 46.15％),with the resist-ance types being predominantly low levels of resistance.Resistance to boscalid of B.cinerea was dif-ferent in various regions.Among these regions,the resistance frequencies of B.cinerea isolates in Jin-nan,Lüliang,and Taiyuan regions were all greater than 50％.However,the resistance frequencies in Xinzhou and Yangquan regions were lower,with 20.00％and 10.81％,respectively.Resistance mech-anism analysis showed that all nine resistant isolates had single point mutations,including P225F,N230I,and H272R.Additionally,the mycelial growth rate,sclerotinia production ability and patho-genicity of the resistant isolates were not significantly different from those of the susceptible isolates,although the spore production decreased significantly.No cross-resistance was observed between boscalid and pyraclostrobin,difenoconazole,pyrimethanil,or prochloraz.The resistance of B.cinerea to boscalid in protected vegetables of Shanxi province was continuously increasing,with the biological fitness of resistant isolates being similar to that of susceptible isolates.Therefore,the use of boscalid should be limited during the later stages of gray mold prevention,and the four fungicides-pyra-clostrobin,difenoconazole,pyrimethanil,and prochlorazare recommended for rotational or combined use.关键词
Botrytis cinerea/啶酰菌胺/抗药性/SdhB基因/生物适合度/交互抗性Key words
Botrytis cinerea/Boscalid/Drug resistance/SdhB gene/Biological fitness/Cross-resist-ance引用本文复制引用
王潞伟,魏甜甜,任潇妍,王春伟,王燕,张作刚,姚艳平,王美琴..山西省蔬菜Botrytis cinerea对啶酰菌胺的抗性检测及生物学性状[J].西北农业学报,2025,34(1):169-176,8.基金项目
山西农业大学产业引领工程项目(CYYL23-25) （CYYL23-25）
山西省现代农业产业技术体系建设项目(2023CYJSTX08-17) （2023CYJSTX08-17）
山西省基础研究计划(自由探索类)面上项目(20210302123404). Industry Leading Project of Shanxi Agricultural University(No.CYYL23-25) （自由探索类）
Con-struction Project of Shanxi Province Modern Agricultural Industry Technology System(No.2023CYJSTX08-17) （No.2023CYJSTX08-17）
Basic Research Program Project of Shanxi Province(Free Exploration Type)(No.20210302123404). （Free Exploration Type）
