西北农业学报2025，Vol.34Issue(1)：184-190,7.DOI:10.7606/j.issn.1004-1389.2025.01.021
民勤青土湖肉苁蓉茎腐病病原菌的分离鉴定
Isolation and Identification of Pathogens Causing Stem Rot of Cistanche deserticola in Qingtu Lake,Minqin
摘要
Abstract
To clarify pathogen species causing stem rot in Cistanche deserticola at Qingtu Lake of Minqin,Gansu Province,the tissue isolation method,morphological characteristics and ITS molecular methods were used for pathogen isolation and identification.The results showed that three different pathogens were isolated from stem rot in C.deserticola at Qingtu Lake,the symptoms observed both in indoor and field were essentially the same,showing typical characteristics of stem rot.These patho-gens were designated as RCRJF-1,RCRJF-2,and RCRJF-3,respectively.The ITS molecular identifica-tion showed that RCRJF-1,RCRJF-2 and RCRJF-3 exhibited 100％similarity to Fusarium prolifera-tum(accession number MT560212),Fusarium oxysporum(accession number MT530243)and Fusar-ium acuminatum(accession number MH341217),respectively.Based on morphological characteris-tics,it was determined that the pathogens causing the stem rot in C.deserticola in Qingtu Lake,Min-qin,Gansu were F.proliferatum,F.oxysporum,and F.acuminatum.The isolation frequencies were 52.38％,31.11％,and 16.51％,respectively,with F.proliferatum identified as the dominant pathogen.关键词
肉苁蓉/茎腐病/病原菌/分离/鉴定Key words
Cistanche deserticola/Stem rot/Pathogen/Separation/Identification引用本文复制引用
刘伟,何彩,金娜,赵连鑫,晋敏,张军,李强,李栋,段爱莉..民勤青土湖肉苁蓉茎腐病病原菌的分离鉴定[J].西北农业学报,2025,34(1):184-190,7.基金项目
甘肃省科技计划(技术创新引导计划)(21CX6NH279) （技术创新引导计划）
甘肃省市场监督管理局科技计划(SSCJG-SP-A202209) （SSCJG-SP-A202209）
中央财政林草科技推广示范项目(甘[2023]ZYTG020号) （甘[2023]ZYTG020号）
甘肃省第二批陇原青年英才([2023]11号). Science and Technology Planof Gansu Province(Technology Innovation Guidance Plan)(No.21CX6NH279) （[2023]11号）
Science and Technology Plan of Market Supervision Administration of Gansu Province(No.SSCJG-SP-A202209) （No.SSCJG-SP-A202209）
Central Finance Forest and Grass Science and Technology Promotion Demonstration Project(No.Gan[2023]ZYTG020) （No.Gan[2023]ZYTG020）
the Second Group of Longyuan Young Talents in Gansu Province(No.[2023]11). （No.[2023]11）
