烟草科技2024，Vol.57Issue(12)：1-14,14.DOI:10.16135/j.issn1002-0861.2024.0421
基于HS-SPME-GC-MS的化学计量学分析有无霉变雪茄烟叶挥发性成分的差异
Chemometric analysis on volatile components and their differences between moldy and non-moldy cigar tobacco leaves using HS-SPME-GC-MS
摘要
Abstract
To investigate the composition,relative contents,and their differences on volatile chemical components between moldy and non-moldy cigar tobacco leaves,a headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)method was established to analyze the volatile components in 18 samples of moldy and non-moldy cigar tobacco leaves.Chemometric methods including principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA)and variable importance in projection were used to identify the main differences in the volatile components in these samples.The results showed that:1)Ninety-five volatile components in the moldy and non-moldy samples were identified and semi-quantified by the established method.2)The volatile components in the moldy and non-moldy samples showed a certain degree of discrimination by PCA.3)The results of identification and analysis using an OPLS-DA model(R2 X=0.74,R2 Y=0.90,Q2=0.60)showed that the moldy and non-moldy cigar leaves were more clustered in their categories,and there was a good degree of separation between the categories,which effectively distinguished the two types of samples.4)There were 22 differential volatile components,such as nicotyrine and β-damascone,with larger contributions(VIP>1)to the model classification in the moldy and non-moldy samples.关键词
雪茄烟叶/霉变/挥发性成分/顶空固相微萃取-气相色谱-质谱(HS-SPME-GC-MS)/化学计量学Key words
Cigar tobacco leaf/Mildew/Volatile component/Headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)/Chemometric分类
轻工纺织引用本文复制引用
魏敏,高云逸,董平,向陈琼,李冉,杨玥皎,黄旭辉,张胜华,余琼卫..基于HS-SPME-GC-MS的化学计量学分析有无霉变雪茄烟叶挥发性成分的差异[J].烟草科技,2024,57(12):1-14,14.基金项目
中国烟草总公司重大科技项目"手工雪茄烟加工过程中霉变生防控制技术研究与应用"[110202201034(XJ-05)] （XJ-05）
国家自然科学基金面上项目"食品中苯并咪唑类农兽药残留的高通量、高选择性、高灵敏度分析新方法及应用研究"(31671929) （31671929）
湖北中烟工业有限责任公司科技项目"手工雪茄霉变微生物分析及防控技术应用研究"(2023JCZL2JS2B031). （2023JCZL2JS2B031）
