烟草科技2024，Vol.57Issue(12)：42-48,7.DOI:10.16135/j.issn1002-0861.2024.0287
烟草NtDHQ/SDH8基因突变对卷烟主流烟气中苯酚释放量的影响
Effects of NtDHQ/SDH8 mutations on phenol release in mainstream cigarette smoke
摘要
Abstract
To determine the effects of mutations in 3-dehydroquinic acid dehydrase/shikimate dehydrogenase(DHQ/SDH),an important bifunctional enzyme in the shikimate pathway,on tyrosine synthesis and the release of phenol and other phenolic compounds in mainstream cigarette smoke,fourteen ntdhq/sdh8 mutants were screened and identified by TILLING technology in the EMS mutagenesis population of tobacco(Nicotiana tabacum L.).The mutation sites and the functional variation of the proteins caused by the mutation were analyzed,and the mass fractions of tyrosine,proline,asparagine,and total protein,as well as the release of phenol,o-dihydroxybenzene,p-dihydroxybenzene,m-dihydroxybenzene,m/p-cresol,and o-cresol in mainstream cigarette smoke were determined.The results showed that the tertiary structure of the DHQ functional domain in NtDHQ/SDH8 protein was altered by amino acid variation,which affected the enzymatic activity of the three ntdhq/sdh8 mutants.Compared with the wild-type control,the tyrosine mass fraction in NtDSM03 and NtDSM12 mutants decreased by 76.78%and 81.71%,respectively;and the phenol release from mainstream cigarette smoke of the two mutants decreased by 24.09%and 48.99%,respectively;all of which showed extremely significant differences(P<0.01).In particular,the mass fractions of total protein,tyrosine,and phenolic compounds such as phenol,catechol,m/p-cresol,and o-cresol in the mainstream cigarette smoke of the NtDSM12 mutant decreased extremely significantly compared to the wild-type control(P<0.01).关键词
烟草突变体/3-脱氢奎尼酸脱水酶/莽草酸脱氢酶/酪氨酸/苯酚/酚类化合物/主流烟气Key words
Tobacco mutant/3-Dehydroquinate dehydratase/shikimate dehydrogenase/Tyrosine/Phenol/Phenolic compound/Mainstream cigarette smoke分类
轻工纺织引用本文复制引用
李锋,刘楠,樊武广,姜兴益,王燃,金立锋,李正风,王大伟..烟草NtDHQ/SDH8基因突变对卷烟主流烟气中苯酚释放量的影响[J].烟草科技,2024,57(12):42-48,7.基金项目
中国烟草总公司烟草基因组计划重大专项项目"低烟气苯酚释放量的云烟87、K326、红花大金元定向改良"[110201901019(JY-06)] （JY-06）
中国烟草总公司河南省公司科技重点项目"特征香韵烤烟品系提高浓香型原料工业可用性的应用研究"(2021410000240025). （2021410000240025）
