中国司法鉴定Issue(6)：63-68,6.DOI:10.3969/j.issn.1671-2072.2024.06.008
STRtyper-21G(Plus)试剂盒在法医物证检测中等位基因丢失现象的研究
Analysis of Allele Drop-out in the Application of STRtyper-21G(Plus)Kit in Forensic Genetics
摘要
Abstract
Objective Analyze the drop-out of alleles in the STRtyper-21G(Plus)kit in forensic genetics application,explore its impact on identification results,and propose solutions.Methods Using the STRtyper-21G(Plus)kit,20 autosomal short tandem repeat(STR)loci of 2 540 blood stains were directly amplified.Capillary electrophoresis was used to analyze the genotypes of each locus.For samples containing loci that do not in accordance with Mendelian inheritance,the STRtyper-32G kit was used for validation.Results After verification by the STRtyper-32G kit,it was found that 9 individuals in 5 cases had allelic drop-out detected using the STRtyper-21G(Plus)kit,involving three loci:D7S820,D19S433,and D18S51.Conclusion In the practice of forensic genetics,using any detection system for detecting STRs may result in allele drop-out and inaccurate paternity test results.To ensure the accuracy of paternity test results,other detection systems with the same locus can be used for verification.关键词
法医物证/STRtyper-21G(Plus)试剂盒/等位基因丢失Key words
forensic genetics/STRtyper-21G(Plus)kit/allele drop-out分类
社会科学引用本文复制引用
毛华芬,许天龙,周保成,尹婷,汤欣欣,王雷雷..STRtyper-21G(Plus)试剂盒在法医物证检测中等位基因丢失现象的研究[J].中国司法鉴定,2024,(6):63-68,6.基金项目
连云港市卫生健康科技项目(202331) （202331）
连云港市妇幼健康科研项目(F202308). （F202308）
