安徽农业大学学报2024，Vol.51Issue(6)：943-954,12.DOI:10.13610/j.cnki.1672-352x.20241227.001
板栗'燕山早丰'种实腐烂病致病菌及致病性探究
Identification and pathogenecity of rot fungal pathogens of Castanea mollissima var.Yanshanzaofeng
摘要
Abstract
Castanea mollissima var.Yanshanzaofeng,a significant chestnut variety in China,is perennially affected by a complex fruit/seed rot disease,whose pathogenic composition and virulence have remained unclear.In this study,Koch's postulates were employed to isolate and identify the causative pathogens,combining molec-ular phylogenetic analysis with morphological characteristics.Furthermore,the pathogenicity of fungal conidia,the virulence of endophytic fungi within chestnuts,and the impact of temperature on pathogenicity were evaluated.The results indicated that the pathogens responsible for fruit/seed rot in'Yanshanzaofeng'chestnuts were distrib-uted across several genera,including Botryosphaeria,Diaporthe,Fusarium,Trichothecium,Colletotrichum,Al-ternaria and Lasiodiplodia.Among these,B.dothidea,D.nobilis and Lasiodiplodia species were identified as the primary pathogenic species.It was found that lower temperatures significantly affected the virulence of these pathogens and reduced the diversity of pathogenic species.The conidia of these fungi were determined to be pathogenic,and strains of Diaporthe and Alternaria endophytic fungi within the chestnut also exhibited patho-genicity.This study systematically revealed,for the first time,the pathogenic structure and virulence of a fruit/seed rot disease in a chestnut variety,which lay a crucial foundation for potential exploration and the estab-lishment of effective prevention and control strategies and techniques.关键词
板栗/'燕山早丰'/种实腐烂病/病菌/鉴定Key words
chestnut/variety of Yanshanzaofeng/fruit rot diseases/pathogens/identification分类
农业科技引用本文复制引用
周爽,闾星,刘昊柟,汪慧华,李如华,李志朋,刘建玲,严东辉..板栗'燕山早丰'种实腐烂病致病菌及致病性探究[J].安徽农业大学学报,2024,51(6):943-954,12.基金项目
中国林业科学研究院基本业务费专项(CAFYBB2021ZF001),国家重点研发计划项目(2022YFD401003)和河北省重点研发计划项目(20326508D)共同资助. （CAFYBB2021ZF001）
