安徽医科大学学报2024，Vol.59Issue(12)：2065-2071,7.DOI:10.19405/j.cnki.issn1000-1492.2024.12.001
PDK1在衰老小鼠小脑中的功能研究
Functional study of PDK1 in the cerebellum of aging mice
摘要
Abstract
Objective To investigate the effects of 3-phosphoinositide-dependent protein kinase-1(PDK1)on the purkinje cells(PCs)in aging mice.Methods The footprint test,elevated beam-walk test and rotarod test were used to measure the gait traits,motor balance and motor coordination of mice.Immunoflourescence was used to de-tect the structure of the cerebellum and the morphology of cells in the cerebellum.The expression level of PDK1,protein kinase C gamma(PKCγ)and protein kinase B(also known as AKT)and the phosphorylation of ribosomal protein S6(rpS6)in the cerebellum were detected by Western blot.Results The mice showed motor defects in the overlap between forelimb and hindlimb,motor balance and motor coordination ability in an age-dependent man-ner.Furthermore,the cerebellum and the soma size of the PCs decreased in an age-dependent manner.However,the density of the PCs was not affected by age.Additionally,the expression level of the PDK1,PKCγ,AKT and the phosphorylation of rpS6 in the cerebellum were reduced with age.Conclusion The cerebellar size and the cell soma size of PCs decrease in the old mice.These effects may contribute to the reduction of the expression of PKCγand AKT and the phosphorylation of rpS6 in the old mice,ultimately affecting the cerebellar motor coordination.关键词
衰老/浦肯野细胞/3-磷酸肌醇依赖性蛋白激酶1/核糖体蛋白S6/γ型蛋白激酶C/蛋白激酶BKey words
aging/purkinje cells/PDK1/rpS6/PKCγ/AKT分类
医药卫生引用本文复制引用
彭文婕,齐翠,刘睿,高隽..PDK1在衰老小鼠小脑中的功能研究[J].安徽医科大学学报,2024,59(12):2065-2071,7.基金项目
国家自然科学基金项目(编号:32371053、32100811) （编号:32371053、32100811）
江苏省高等学校自然科学研究项目(编号:21KJB180008) National Natural Science Foundation of China(Nos.32371053,32100811) （编号:21KJB180008）
Natural Science Research Project in Higher Education Institutions of Jiangsu Province(No.21KJB180008) （No.21KJB180008）
