安徽医科大学学报2024，Vol.59Issue(12)：2072-2078,7.DOI:10.19405/j.cnki.issn1000-1492.2024.12.002
罗布麻叶提取物通过下丘脑室旁核改善慢性应激小鼠的焦虑样行为
Improvement of apocynum venetum leaves on anxiety behavior through hypothalamus paraventricular nucleus in chronic stress mice
摘要
Abstract
Objective To observe the effects of apocynum venetum leaves extract(AVLE)on anxiety-like behavior caused by chronic restraint stress(CRS).Methods Male C57BL/6 mice were randomly divided into 4 groups,5 per group:control group(equivalent physiological saline 0.2 ml/10 g),CRS model group(equivalent physiologi-cal saline 0.2 ml/10 g),AVLE treatment group(AVLE 120 mg/kg)and positive control group(paroxetine hydrochloride 3 mg/kg).The anxiety mouse model of chronic restraint stress was established,and the anxiety level of mice treated with apocynum venetum leaf extract was evaluated by open field test and elevated cross maze test.Immunofluorescence staining was used to detect the changes of serotonin receptor in hypothalamic paraventricular nucleus.Chemogenetics combined with adeno-associated virus was used to detect the effect of hypothalamic oxyto-cin neurons on the anxiety regulation of AVLE.Results Compared with the model group,apocynum venetum leav-es extract could increase the total distance of open field exercise,the activity time,the time of mice entering the central area(P＜0.05),and the activity distance and time of mouse elevated maze(P＜0.05).The expression of serotonin 1A receptor in the paraventricular nucleus of hypothalamus was enhanced after treatment with AVLE,and inhibition of serotonin 1A receptor and oxytocin neurons in hypothalamic paraventricular nucleus could decrease ac-tivity level in mice(P＜0.05).Conclusion AVLE can improve the anxiety state and activity ability of CRS mice,and its effect is related to serotonin receptor and oxytocin neurons in hypothalamic paraventricular nucleus.关键词
罗布麻叶/焦虑/下丘脑/五羟色胺受体/神经元/慢性束缚应激Key words
apocynum venetum leaf/anxiety/hypothalamus/serotonin receptors/neuron/chronic restraint stress分类
医药卫生引用本文复制引用
杜桐宇,李群涛,殷洁婷,殷姜文,李燕..罗布麻叶提取物通过下丘脑室旁核改善慢性应激小鼠的焦虑样行为[J].安徽医科大学学报,2024,59(12):2072-2078,7.基金项目
国家自然科学基金项目(编号:82360242、81860209) National Natural Science Foundation of China(Nos.82360242,81860209) （编号:82360242、81860209）
