安徽医科大学学报2024，Vol.59Issue(12)：2094-2102,9.DOI:10.19405/j.cnki.issn1000-1492.2024.12.005
原肌球调节蛋白3在肝细胞癌复发转移中的潜在作用分析
Analysis of the potential role of TMOD3 in recurrence and metastasis of hepatocellular carcinoma
摘要
Abstract
Objective To investigate the relationship between tropomodulin 3(TMOD3)and the malignant biologi-cal characteristics of hepatocellular carcinoma,and the predictive potential of TMOD3 as a biomarker for the recur-rence and metastasis of hepatocellular carcinoma.Methods Firstly,the structure of TMOD3 and its subcellular lo-calization in cells and tissues were analyzed using database of Human Protein Atlas.Then explored the differential expression of TMOD3 in hepatocellular carcinoma tissues and normal liver tissues and its impact on clinical patho-logical characteristics and prognosis using TCGA and GEO datasets.Subsequently,the STRING database was uti-lized to explore the interacting proteins of TMOD3,followed by enrichment analysis conducted using the Metascape database.Finally,Logistic regression was used to analyze the independent risk factors for metastasis of hepatocellu-lar carcinoma and evaluated the importance of predictive variables using ROC curves and Wald tests.Survival anal-ysis was conducted using the Kaplan-Meier curve and the Log-rank test.Results TMOD3 was localized to actin filaments in cells,and compared with normal tissues,the expression level of TMOD3 in liver cancer tissues is high-er(P＜0.05),and high expression of TMOD3 was closely related to lymph node metastasis and distant metastasis of hepatocellular carcinoma patients(P＜0.05).Enrichment analysis results revealed that TMOD3 and its interac-ting proteins mainly function and signaling pathways related to tumor invasion and migration.Logistic regression found that TMOD3 was an independent risk factor for recurrence and metastasis of hepatocellular carcinoma(OR:4.359,95％CI:1.235-15.384,P=0.022).Survival analysis revealed that high expression of TMOD3 was as-sociated with poor OS,DFS,and RFS of hepatocellular carcinoma patients(P＜0.05).Both ROC analysis and Wald test indicated that TMOD3 has good predictive characteristics for recurrence and metastasis of hepatocellular carcinoma.Conclusion TMOD3 is closely associated with the invasion and metastasis of hepatocellular carcinoma and is an independent risk factor for the recurrence and metastasis of liver cancer.TMOD3 performs well in predic-ting the recurrence and metastasis of hepatocellular carcinoma and has the potential to become a biomarker for pre-dicting the recurrence and metastasis of hepatocellular carcinoma.关键词
原肌球调节蛋白3/肝细胞癌/转移/组织芯片/生物标志物/生物信息学Key words
tropomodulin 3/hepatocellular carcinoma/metastasis/tissue microarray/biomarker/bioinformatics分类
医药卫生引用本文复制引用
罗龙彪,蒋辉,马丹丹,付航玮,孙炜玮..原肌球调节蛋白3在肝细胞癌复发转移中的潜在作用分析[J].安徽医科大学学报,2024,59(12):2094-2102,9.基金项目
国家自然科学基金项目(编号:82300716) National Natural Science Foundation of China(No.82300716) （编号:82300716）
