安徽医科大学学报2024，Vol.59Issue(12)：2112-2116,5.DOI:10.19405/j.cnki.issn1000-1492.2024.12.007
基于IP3-蛋白激酶C通路探讨人视网膜血管内皮细胞高糖性损伤的作用机制
Exploring the mechanism of high glucose-induced damage of human retinal vascular endothelial cells based on the IP3-protein kinase C pathway
摘要
Abstract
Objective To explore the damaging effect of the IP3-protein kinase C pathway on human retinal vascu-lar endothelial cells under high glucose conditions.Methods Human retinal vascular endothelial cells in the loga-rithmic growth phase were taken and divided into the experimental group(10 mmol/L glucose and 20 mmol/L glu-cose)and the control group(5 mmol/L glucose).The morphological changes of cells were observed under different concentrations of glucose culture medium.The apoptosis of human retinal vascular endothelial cells was detected by flow cytometry.Real-time fluorescent quantitative reverse transcription polymerase chain reaction was used to detect the RNA expression level of PKC in the IP3-protein kinase C(PKC)pathway,and Western blot was used to detect the protein expression level of PKC in cells.Results Under high glucose conditions,the volume of human retinal vascular endothelial cells increased,the degree of extension decreased,and the apoptosis rate increased.Compared with the control group,the apoptosis rate of human retinal vascular endothelial cells in the 20 mmol/L glucose group increased(P＜0.05);the IP3 level of human retinal vascular endothelial cells in the 20 mmol/L glucose group(587.9±15.2)ng/ml was lower than that of the control group(738.9±1.0)ng/ml(P＜0.05).Under high glucose treatment,the expression levels of PKC mRNA and protein increased(P＜0.05).Conclusion The IP3-PKC pathway may be involved in the damage process of human retinal vascular endothelial cells under high glu-cose conditions,and thus may play a role in diabetic retinopathy.关键词
人视网膜血管内皮细胞/IP3-蛋白激酶C通路/糖尿病视网膜病变/高糖/凋亡Key words
human retinal vascular endothelial cells/IP3-protein kinase C pathway/diabetic retinopathy/high glucose/apoptosis分类
医药卫生引用本文复制引用
刘强,俞华,董立红,廖荣丰..基于IP3-蛋白激酶C通路探讨人视网膜血管内皮细胞高糖性损伤的作用机制[J].安徽医科大学学报,2024,59(12):2112-2116,5.基金项目
安徽省高校科研项目(编号:2023AH050643) （编号:2023AH050643）
安徽医科大学校科研基金项目(编号:2022xkj063) Scientific Research Project of Universities in Anhui Province(No.2023AH050643) （编号:2022xkj063）
Scientific Research Project of Anhui Medical University(No.2022xkj063) （No.2022xkj063）
