安徽医科大学学报2024，Vol.59Issue(12)：2127-2134,8.DOI:10.19405/j.cnki.issn1000-1492.2024.12.009
敲低E2F1抑制人舌鳞癌细胞增殖及迁移的机制研究
The mechanism of knockdown of E2F1 inhibiting the proliferation and migration of human tongue squamous cell carcinoma cells
摘要
Abstract
Objective To investigate the effects of knockdown of E2F transcription factor 1(E2F1)in human tongue squamous cell carcinoma cells on proliferation,migration,and invasion of tongue squamous cell carcinoma cells,and to explore its possible mechanisms.Methods The expression of E2F1 in human tongue squamous cell carcinoma was detected by RT-PCR,and the background expression of E2F1 gene in HOEC,SCC-9,CAL-27,TSCCa and SCC-25 cells was detected by RT-PCR,and the cell lines with significantly high expression of E2F1 were screened for subsequent experiments.CCK-8,EdU,colony formation,cell scratch,Transwell,apoptosis and cell cycle experiments were used to detect the effects of knockdown of E2F1 on proliferation,migration,invasion and apoptosis of human tongue squamous cell carcinoma cells after knockdown of E2F1 gene in tongue squamous cell carcinoma candidate cells.Western blot was used to detect the expression of epithelial mesenchymal transition markers E-cadherin,N-cadherin and snail family transcription inhibitor 1(Snail)protein and WNT signaling path-way markers WNT family members 3A(WNT3A),β-catenin and cyclin D1(CCND1).Results RT-PCR results showed that the expression of E2F1 in cancer tissues was significantly higher than that in adjacent tissues(P＜0.001).At the same time,compared with HOEC cells,the expression of E2F1 in SCC-9,CAL-27,TSCCa and SCC-25 cells significantly increased(P＜0.05),and the high expression in SCC-25 cells was the most obvious.SCC-25 would be used as an experimental cell line in subsequent experiments.After knocking down E2F1 in SCC-25 cells,the viability of CCK-8 cells was significantly inhibited(P＜0.001).The number of EdU positive cells decreased significantly(P＜0.001).The number of cell clones was significantly reduced(P＜0.001).The pro-portion of total apoptosis significantly increased(P＜0.001).The proportion of cells in G1 phase increased(P＜0.01).The proportion of cells in G2 phase decreased(P＜0.01).The cell migration and invasion ability were sig-nificantly inhibited(P＜0.001).Western blot showed that the expression of WNT signaling pathway proteins WNT3A,β-catenin and CCND1 decreased(P＜0.001),while the expression of EMT-related proteins N-cadherin and Snail decreased(P＜0.001),while the expression of E-cadherin increased(P＜0.001).Conclusion Knockdown of E2F1 inhibits the proliferation,migration,invasion and EMT process of human tongue squamous cell carcinoma SCC-25 cells,and promotes apoptosis.This anti-tumor effect may be achieved by blocking the activation of WNT signaling pathway.关键词
E2F1/舌鳞癌/增殖/迁移/侵袭/凋亡/EMT/WNTKey words
E2F1/tongue squamous cell carcinoma/proliferation/migration/invasion/apoptosis/EMT/WNT分类
医药卫生引用本文复制引用
杜明珠,张斌..敲低E2F1抑制人舌鳞癌细胞增殖及迁移的机制研究[J].安徽医科大学学报,2024,59(12):2127-2134,8.基金项目
辽宁省教育厅科学技术研究项目(编号:JYTJCZR2020062) Science and Technology Research Project of Liaoning Provincial Department of Education(No.JYTJCZR2020062) （编号:JYTJCZR2020062）
