安徽医科大学学报2024，Vol.59Issue(12)：2135-2141,7.DOI:10.19405/j.cnki.issn1000-1492.2024.12.010
创伤后应激障碍大鼠海马区炎症激活及髓鞘改变
Inflammatory activation and myelin alterations in the hippocampus of post-traumatic stress disorder rats
摘要
Abstract
Objective To investigate the alterations in hippocampal neurons,nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome,microglia,and myelin in rats with post-traumatic stress disorder(PTSD).Methods A PTSD rat model was established using the single prolonged stress(SPS)para-digm.Anxiety and cognitive functions were evaluated through the open field test,elevated plus maze,and Morris water maze.Histopathological changes in hippocampal neurons were assessed using hematoxylin and eosin(HE)staining.The expression levels of NLRP3 and iba-1(a microglial marker)in the hippocampus were examined using immunofluorescence staining.Immunohistochemical staining and Luxol Fast Blue staining were performed to investi-gate alterations in hippocampal myelin.Results Hippocampal neurons in PTSD rats exhibited damage,with in-creased activation of the NLRP3 inflammasome and microglia,and elevated myelin content.Conclusion The pathogenesis of PTSD may be associated with hippocampal neuronal damage,inflammatory responses,and changes in myelin.关键词
创伤后应激障碍/海马/NLRP3/髓鞘/神经元/小胶质细胞Key words
post-traumatic stress disorder/hippocampus/NLRP3/myelin/neurons/microglia分类
医药卫生引用本文复制引用
杨罗东,石岩,张子维,李斌,张桂青..创伤后应激障碍大鼠海马区炎症激活及髓鞘改变[J].安徽医科大学学报,2024,59(12):2135-2141,7.基金项目
国家自然科学基金项目(编号:32260208) National Natural Science Foundation of China(No.32260208) （编号:32260208）
