安徽医科大学学报2025，Vol.60Issue(1)：1-9,9.DOI:10.19405/j.cnki.issn1000-1492.2025.01.001
红景天仿生纳米药物对急性胰腺炎大鼠的胰腺保护作用及其机制
Protective effect of Rhodiola rosea bionic nanomedicine on pancreas of acute pancreatitis rats and its mechanism
摘要
Abstract
Objective To explore the protective effect and mechanism of Rhodiola rosea(Rho)bionic nanomedi-cine in rats with acute pancreatitis.Methods Erythrocyte membrane vesicles(EMV)were used as biomimetic nanomedicine coating materials to construct Rho biomimetic nanomedicine.Meanwhile,rat models of acute pancre-atitis were constructed and divided into acute pancreatitis model(AP)group,Rho group,EMV group and Rhodio-la rosea erythrocyte membrane vesicles(R-EMV)group.R-EMV group rats were further treated with NLRP3 acti-vator BMS-986299(R-EMV+BMS-986299 group)and inhibitor MCC950(R-EMV+MCC950 group).The chan-ges of abdominal water volume were observed,and serum levels of interleukin(IL-6),IL-1β,endothelin(ET),diamine oxidase(DAO),malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)were detected by enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE)staining was used to observe the pathological changes of pancreatic tissue.Immunohistochemistry,reverse transcription-polymerase chain reac-tion(RT-PCR)and Western blot were used to analyze the expression of NOD-like receptor protein 3(NLRP3)and nuclear transcription factor-Kappa B(NF-κB)in pancreatic tissue.Results The prepared R-EMVs were nearly circular,the average particle size was(244.61±1.08)nm,and the Zeta potential was-(11.13±1.25)mV.At the same time,the EMV could load(58.67±0.79)μg of Rho.Compared with Sham group,the abdominal water volume of rats in AP group was significantly higher(t=33.79,P＜0.01),and the levels of peripheral blood immune indexes IL-1 β,IL-6,ET and DAO increased(t=38.25,42.54,29.20,34.92,all P＜0.01).In AP group,there were obvious tissue hyperemia and edema,widening of lobular space,infiltration of a large number of inflammatory cells,and increased pancreatic pathological score(t=30.06,P＜0.01).Compared with AP group,abdominal water volume,dry-wet weight ratio of pancreas,pathological score,amylase and lipase levels in Rho group decreased(F=1 523.7,543.3,839.9,446.1,172.2,P＜0.05).Compared with other groups,the lev-els of serum IL-1 β,IL-6,ET and DAO in AP group decreased to some extent.Compared with AP group,NF-κB p65 and NLRP3 protein levels and NF-κB and NLRP3 immunohistochemical staining scores decreased in R-EMV group(t=24.54 and 26.91,both P＜0.001).At the same time,the levels of serum IL-1 β,IL-6,ET,DAO,MDA,SOD and GSH in R-EMV+MCC950 group were significantly lower than those in AP group,R-EMV group and R-EMV+BMS-986299 group.Conclusion R-EMV has a good pancreatic protection effect in acute pancreati-tis,which is related to reducing the activity of NF-κB/NLRP3 pathway and down-regulating the expression of in-flammatory factors such as IL-6 and oxidative stress indicators such as MDA.关键词
红景天/仿生纳米/急性胰腺炎/胰腺损伤/大鼠/机制Key words
Rhodiola rosea/biomimetic nanometer/acute pancreatitis/pancreatic injury/rat/mechanism分类
医药卫生引用本文复制引用
张彤哲,赵喜容,李培武..红景天仿生纳米药物对急性胰腺炎大鼠的胰腺保护作用及其机制[J].安徽医科大学学报,2025,60(1):1-9,9.基金项目
国家自然科学基金(编号:82260135) National Natural Science Foundation of China(No.82260135) （编号:82260135）
