安徽医科大学学报2025，Vol.60Issue(1)：10-14,5.DOI:10.19405/j.cnki.issn1000-1492.2025.01.002
改良组织块培养法提取原代大鼠脑微血管内皮细胞及鉴定
Extraction and identification of primary rat brain microvascular endothelial cells by improved tissue block culture method
摘要
Abstract
Objective To investigate the brain microvascular tissue block culture method for extracting primary rat brain microvascular endothelial cells and identify its effect.Methods Brain tissue from 4-week-old Sprague Daw-ley rats was screened,pre-digested and solidified to obtain brain microvascular segments.These segments were subsequently placed in a CO2 incubator for primary culture.The target cells were identified by cell morphology and immunocytochemical staining for factor Ⅷ-related antigen.Results After a 48-hour culture period in vitro,the short spindle cells crawled out from around the brain microvascular segments.After 72 hours,island-like cell cul-sters formed.After 96 hours the clusters fused and the cells formed a typical monolayer,cobble stone-like,and mosaic arrangement.Factor Ⅷ-related antigen immunocytochemical staining showed that the cytoplasm of the cells appeared brown-red,indicating positive expression;DAB stained the nucleus,showing blue-dark.Conclusion The brain microvascular tissue block culture method can isolate and culture primary rat brain microvascular endo-thelial cells.关键词
脑/微血管内皮细胞/原代培养/脑微血管组织块培养法/第Ⅷ因子相关抗原/大鼠/鉴定Key words
brain/microvascular endothelial cells/primary culture/brain microvascular tissue block culture/fac-tor Ⅷ-related antigen/rat/identification分类
医药卫生引用本文复制引用
张帆,李柏霖,池茗,刘海琴,唐元瑜..改良组织块培养法提取原代大鼠脑微血管内皮细胞及鉴定[J].安徽医科大学学报,2025,60(1):10-14,5.基金项目
国家自然科学基金(编号:81072714) （编号:81072714）
福建省自然科学基金(编号:2017J01545) National Natural Science Foundation of China(No.81072714) （编号:2017J01545）
Natural Science Foundation of Fujian Province(No.2017J01545) （No.2017J01545）
