安徽医科大学学报2025，Vol.60Issue(1)：41-48,8.DOI:10.19405/j.cnki.issn1000-1492.2025.01.006
LncRNA SFTA1P通过调控miR-182-5p/FN1通路促进肾透明细胞癌细胞增殖与迁移
LncRNA SFTA1P modulates the miR-182-5p/FN1 pathway to promote the proliferation and migration of clear cell renal carcinoma cells
摘要
Abstract
Objective To explore the molecular mechanism by which long non-coding RNA Surfactant Associated 1 Pseudogene(SFTA1P)promotes the proliferation and migration of clear cell renal cell carcinoma(ccRCC)cells by regulating the microRNA-182-5p(miR-182-5p)/fibronectin 1(FN1)pathway.Methods GEPIA2 software was utilized to analyze the expression of SFTA1P in ccRCC tissues from the TCGA database.Quantitative real-time PCR(qPCR)was employed to detect the expression of SFTA1P in ccRCC tissues,normal kidney tissues and ccRCC cell lines.A subcellular localization experiment was performed to explore the localization of SFTA1P within the hu-man renal cell adenocarcinoma cell line(ACHN)derived from ccRCC.ACHN cells were then divided into the fol-lowing groups:si-Con group,si-SFTA1P #2 group,mimic NC group,miR-182-5p mimic group,anti-miR-Con group,anti-miR-182-5p group,anti-miR-182-5p+si-FN1 group,si-Con+anti-miR-Con group,si-SFTA1P #2+anti-miR-Con group,and si-SFTA1P #2+anti-miR-182-5p group.CCK-8 and Transwell chamber experiments were conducted to assess cell proliferation and migration abilities.qPCR,Western blot,and dual-luciferase reporter as-says were employed to elucidate the regulatory interactions among SFTA1P,miR-182-5p,and FN1.Results A-nalysis of The Cancer Genome Atlas(TCGA)database indicated that SFTA1P was overexpressed in ccRCC tissues(P＜0.05).When compared to normal kidney tissues,SFTA1P expression was markedly elevated in ccRCC tis-sues(P＜0.01).Furthermore,the expression levels of SFTA1P in ccRCC cell lines 786-O,SN12-PM6,ACHN,and A498 were significantly higher than those in human renal proximal tubule cells(HK-2)(all P＜0.01).Sub-cellular localization experiments revealed that SFTA1P predominantly localized in the cytoplasm of ACHN cells.Compared to the si-Con group,the si-SFTA1P #2 group exhibited a significant reduction in proliferation and migra-tion abilities of ACHN cells,accompanied by a decrease in FN1 mRNA and protein expression(P＜0.05).Com-pared to the mimic NC group,the expression of FN1 mRNA and protein in ACHN cells in the miR-182-5p mimic group reduced(P＜0.01).In comparison to the anti-miR-Con group,the expression levels of FN1 mRNA and protein in ACHN cells were significantly elevated in the anti-miR-182-5p group.Additionally,there was a signifi-cant enhancement in both cell proliferation and migration capabilities(P＜0.05).Conversely,the proliferation and migration abilities of ACHN cells in the anti-miR-182-5p+si-FN1 group were significantly reduced compared to the anti-miR-182-5p group(P＜0.05).Furthermore,relative to the si-SFTA1P #2+anti-miR-Con group,the ACHN cells in the si-SFTA1P #2+anti-miR-182-5p group demonstrated increased proliferation and migration abili-ties,along with elevated FN1 mRNA and protein expression levels(P＜0.05).Conclusion SFTA1P exhibits el-evated expression levels in ccRCC and facilitates the proliferation and migration of ccRCC cells through the modula-tion of the miR-182-5p/FN1 signaling pathway.关键词
肾透明细胞癌/SFTA1P/miR-182-5p/FN1/增殖/迁移Key words
clear cell renal cell carcinoma/SFTA1P/miR-182-5p/FN1/proliferation/migration分类
医药卫生引用本文复制引用
向威,吕磊,郑福鑫,袁敬东..LncRNA SFTA1P通过调控miR-182-5p/FN1通路促进肾透明细胞癌细胞增殖与迁移[J].安徽医科大学学报,2025,60(1):41-48,8.基金项目
国家自然科学基金(编号:81502204) （编号:81502204）
湖北省卫生健康委员会科研项目(编号:WJ2021Q001) National Natural Science Foundation of China(No.81502204) （编号:WJ2021Q001）
Scientific Research Project of Hubei Health Commission(No.WJ2021Q001) （No.WJ2021Q001）
