安徽医科大学学报2025，Vol.60Issue(1)：59-65,7.DOI:10.19405/j.cnki.issn1000-1492.2025.01.008
积雪草苷对异丙肾上腺素诱导小鼠心肌损伤的保护作用
Protective effect of asiaticoside on isoproterenol induced myocardial injury in mice
摘要
Abstract
Objective To study the protective effect of asiaticoside(AS)on Isoproterenol Hydrochloride(ISO)-induced myocardial injury in mice.Methods Sixty male C57BL/6 mice were randomly divided into blank control(CON)group,model group[ISO,ISO 10/(kg·d)],Low dose group[ISO+AS-L,ISO 10 mg/(kg·d)+AS 5 mg/(kg·d)],Medium dose group[ISO+AS-M,ISO 10 mg/(kg·d)+AS 10 mg/(kg·d)],High dose group[ISO+AS-H,ISO 10 mg/(kg·d)+AS 20 mg/(kg·d)].Heart mass ratio was counted;changes were observed in electrocardiogram;Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of inter-leukin(IL)-1β and cardiac troponin T(cTn-T)in serum;Masson staining was used to observe the fibrosis of mouse myocardial tissue;Western blot was used to detect the ratio of Bax and Bcl-2 protein expression levels(Bax/Bcl-2)and the expression levels of Caspase-3 and NLRP3 proteins in myocardial tissue;real-time quantitative poly-merase chain reaction(qPCR)was used to detect the mRNA expression levels of ANP,BNP,β-MHC,TNF-α,IL-6,Type Ⅰ collagen(COL Ⅰ),and Type Ⅲ collagen(COL Ⅲ).Results Compared with the CON group,the ISO group had an elevated heart-to-mass ratio(P＜0.01),a lower heart rate(P＜0.05),a prolonged QT in-terval(P＜0.05),elevated expression of myocardial injury markers cTn-T,ANP,BNP,andβ-MHC(P＜0.01);increased expression of IL-1 β in the serum(P＜0.01),increased expression of TNF-α in the cardiac tissue and increased IL-6 expression(P＜0.001),and NLRP3 protein expression was elevated(P＜0.05);myocardium showed a large number of collagen fibers bluish staining(P＜0.001),COL Ⅰ,COL Ⅲ mRNA expression levels increased(P＜0.001),and Bax/Bcl-2 ratio(P＜0.001)and Caspase-3 expression were significantly elevated(P＜0.05).Compared with ISO group,heart-to-mass ratio of mice in ISO+AS-L and ISO+AS-M groups decreased(P＜0.05),heart rate increased,QT interval was shortened,cTn-T,ANP,BNP and β-MHC decreased(P＜0.001),myocardial collagen fiber blue-staining decreased(P＜0.01).The mRNA expression levels of COL Ⅰand COL Ⅲ decreased(P＜0.05).The expression levels of IL-1 β and TNF-α decreased(P＜0.01).NLRP3,Caspase-3 protein expression and Bax/Bcl-2 ratio decreased(P＜0.05).The expression level of IL-6 in ISO+AS-M group decreased(P＜0.01).The expression levels of ANP,BNP,and TNF-α mRNA expression were reduced in the ISO+AS-H group(P＜0.001);the degree of myocardial fibrosis was improved(P＜0.05),and the ex-pression levels of COL Ⅰ and COL Ⅲ mRNA were reduced(P＜0.05).Conclusion AS has a protective effect a-gainst ISO-induced myocardial injury in mice by ameliorating cardiac fibrosis,inhibiting cardiomyocyte apoptosis and attenuating myocardial tissue inflammatory response.关键词
积雪草苷/异丙肾上腺素/心肌损伤/凋亡/炎症/纤维化/保护作用Key words
asiaticoside/isoproterenol/myocardial injury/apoptosis/inflammation/fibrosis/protective function分类
医药卫生引用本文复制引用
马子雨,左芯萌,王振宇,王明明,李翠..积雪草苷对异丙肾上腺素诱导小鼠心肌损伤的保护作用[J].安徽医科大学学报,2025,60(1):59-65,7.基金项目
国家自然科学基金(编号:81500177) （编号:81500177）
江苏省自然科学基金(编号:BK20150211) （编号:BK20150211）
江苏省研究生科研创新计划(编号:KYCX23_2942) National Natural Science Foundation of China(No.81500177) （编号:KYCX23_2942）
Natural Science Foundation of Jiangsu Province(No.BK20150211) （No.BK20150211）
Graduate Student Research Innovation Project of Jiangsu Province(No.KYCX23_2942) （No.KYCX23_2942）
