安徽医科大学学报2025，Vol.60Issue(1)：73-78,6.DOI:10.19405/j.cnki.issn1000-1492.2025.01.010
白藜芦醇对类风湿性关节炎患者成纤维样滑膜细胞cGAS-STING信号通路的影响
Effects of resveratrol on cGAS-STING signaling pathway in fibroblast-like synoviocytes of patients with rheumatoid arthritis
摘要
Abstract
Objective To investigate the effects of resveratrol(Res)on fibroblast-like synoviocytes(FLS)in pa-tients with rheumatoid arthritis(RA),and to explore the possible mechanism of Res inhibiting the release of in-flammatory factors from FLS.Methods FLS from RA patients were cultured in vitro and treated with different con-centrations of Res(0,20,40,80,160,320μmol/L).The viability of FLS cells was detected by CCK-8 assay after 12 and 24 h.The contents of inflammatory factor interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in cell supernatant were detected by ELISA.The expression levels of cyclic guanosine monophosphate-adenosine monophosphate synthase(cGAS)and stimulator of interferon gene(STING)were measured by Western blot;After lentivirus infection with FLS caused the cells to overexpress cGAS,the cells were divided into Control group(blank control),cGAS group(cGAS overexpression),Res+cGAS group(Res 160 μmol/L+cGAS overexpression)and Res group(Res 160 µmol/L).The expression level of STING protein in cells of each group was determined by Western blot,the viability of FLS cells in each group was detected by CCK-8,and the contents of inflammatory fac-tor IL-6 and TNF-α in the supernatant of cells of each group were detected by ELISA method.Results The results of CCK-8 experiment showed that under 40,80,160 μmol/L Res treatment,FLS viability decreased significantly after 24 h compared with blank control group(P＜0.01).ELISA results showed that the contents of IL-6 and TNF-α in cell supernatant were also significantly decreased after treatment with Res of 40,80 and 160 μmol/L(P＜0.01).Meanwhile,Western blot results showed that Res could significantly decrease the protein expression lev-els of STING and cGAS in FLS cells after treatment of 40,80 and 160μmol/L(P＜0.05,P＜0.01).Compared with the Control group,the expression level of STING protein in FLS increased after overexpression of cGAS(P＜0.05);compared with the Res group,the content of inflammatory factors in the supernatant of FLS and the expres-sion level of STING protein in FLS significantly increased after overexpression of cGAS(P＜0.01,P＜0.05).Conclusion The appropriate concentration of Res can inhibit the release of inflammatory cytokines in FLS cells,which may be related to the blocking of cGAS-STING signaling pathway.关键词
类风湿性关节炎/成纤维样滑膜细胞/白藜芦醇/cGAS-STING信号通路/白介素-6/肿瘤坏死因子-αKey words
rheumatoid arthritis/fibroblast-like synoviocytes/resveratrol/cGAS-STING signaling pathway/inter-leukin-6/tumor necrosis factor-α分类
医药卫生引用本文复制引用
汪陶荣,邵玉宝,刘楠楠,李文昊,李梦,陈晓宇..白藜芦醇对类风湿性关节炎患者成纤维样滑膜细胞cGAS-STING信号通路的影响[J].安徽医科大学学报,2025,60(1):73-78,6.基金项目
安徽省高校科研计划项目(编号:2023AH050607) （编号:2023AH050607）
安徽医科大学校科研基金(编号:2021xkj004) Natural Science Research Project of Anhui Educational Committee(No.2023 AH050607) （编号:2021xkj004）
Sci-entific Research Project of Anhui Medical University(No.2021xkj004) （No.2021xkj004）
