阿尔茨海默病及相关病2025，Vol.8Issue(1)：36-46,11.DOI:10.3969/j.issn.2096-5516.2025.01.006
元宝枫籽油对阿尔茨海默病模型小鼠的神经保护作用及机制研究
Study on the neuroprotective effect and mechanism of Acer truncatum Bunge seed oil on mouse model of Alzheimer's disease
摘要
Abstract
Objective:The aim of this study was to explore the preventive and curative effect and mechanisms of Acer truncatum Bunge seed oil(ASO)on Alzheimer's disease using 5×FAD transgenic mice as an animal model.Methods:1-month-old male 5×FAD transgenic mice were randomly selected and fed a standard diet supplemented with 4%ASO for 5 months(AD+ASO group),Male wild type control group(WT group)and a 5×FAD transgenic Alzheimer's disease model group(AD group)were also included.Before sampling,animal behavioral experiments such as Barnes maze and elevated plus maze were conducted,followed by immunofluorescence staining of the hippocampus and cerebral cortex,high-throughput 16S rRNA sequencing,and bioinformatics analysis of gut microbiota.Results:Behavioral results showed that ASO intervention could improve the spatial learning and memory and ameliorate cognitive impairment of 5×FAD transgenic mice.Immunofluorescence histological results showed that ASO reduced the deposition of neuroinflammatory plaques formed by beta-amyloid peptide(Aβ)accumulation in the hippocampus and cerebral cortex by inhibiting the formation of amyloid plaques.ASO supplementation increased level of the antioxidant enzyme-glutathione peroxidase(GSH-Px)in the hippocampal tissue of AD mice,which in turn increased the body's antioxidant capacity.Bioinformatics results showed that the ASO could improve the richness and diversity of the gut microbiota in AD mice and play a positive role in regulating the gut microbiota.Conclusion:ASO can inhibit the formation of Aβ amyloid plaques by regulating the structure and abundance of gut microbiota,improve the learning and memory of AD mice and exert its neuroprotective effect,provides new materials and possibilities for protection of AD.关键词
元宝枫籽油/阿尔茨海默病/肠道菌群/淀粉样斑块Key words
Acer truncatum Bunge seed oil/Alzheimer's disease/Gut microbiota/Amyloid plaque分类
化学化工引用本文复制引用
任多多,向晨瑶,赵永康,柴学军,张露露,张玉婵,朱晓岩,赵善廷..元宝枫籽油对阿尔茨海默病模型小鼠的神经保护作用及机制研究[J].阿尔茨海默病及相关病,2025,8(1):36-46,11.基金项目
陕西省科技厅农业类一般项目(2023-YBNY-188) （2023-YBNY-188）
西安医学院高层次引进人才基金(No.2018RCYJ04) （No.2018RCYJ04）
四川省"天府学者"项目(2020-120) （2020-120）
