广西林业科学2024，Vol.53Issue(6)：785-792,8.DOI:10.19692/j.issn.1006-1126.20240613
臭常山提取物体外抑菌作用及活性成分鉴定
In vitro Antibacterial Effects and Identification of Active Components in Orixa japonica Extracts
摘要
Abstract
To investigate in vitro antibacterial effects of Orixa japonica extracts and their active components,O.japonica was used as experimental material.Extracts and water-soluble substance were obtained by using 60%ethanol solution,petroleum ether,ethyl acetate and acetone.Water extract of O.japonica was prepared.Anti-bacterial activity of extracts from different solvents was evaluated by disk diffusion(K-B method).Active com-ponents were identified by mass spectrometry(MS).Results showed that O.japonica extracts from different sol-vents did not exhibit obivious inhibitory effects against Escherichia coli,Pseudomonas aeruginosa,Enterococcus faecalis,Proteus vulgaris and Klebsiella pneumoniae.60%ethanol extract,ethyl acetate extract and acetone ex-tract had relative good inhibitory effects against Staphylococcus aureus.60%ethanol extract exhibited the best inhibitory effect against S.aureus with inhibition zone diameter of 14.00 mm.Minimum inhibitory concentration(MIC)of ethyl acetate extract was 0.25 g/mL.60%ethanol extract showed certain inhibitory effects against Fu-sarium avenaceum,Fusarium oxysporum,and Fusarium proliferatum with average inhibition rate of 16.67%.14 compounds were identified in ethyl acetate extract,and antibacterial effects of ethyl acetate extact may be relat-ed to various alkaloids in extract.关键词
化学成分/抑菌活性/抗真菌活性/臭常山Key words
chemical component/antibacterial activity/antifungal activity/Orixa japonica分类
医药卫生引用本文复制引用
罗正才,胡祥,秦丽,张旭..臭常山提取物体外抑菌作用及活性成分鉴定[J].广西林业科学,2024,53(6):785-792,8.基金项目
毕节市科学技术局联合基金项目(毕科联合字sy[2022]3号) （毕科联合字sy[2022]3号）
