贵州农业科学2025，Vol.53Issue(1)：76-82,7.DOI:10.3969/j.issn.1001-3601.2025.01.010
玛瑙红樱桃不同发育时期胚败育果实转录组分析
Transcriptomic Analysis on Embryo Abortion Fruit of Manaohong Cherry During Different Development Stages
摘要
Abstract
[Objective]The expression differences in related genes during the development process of embryo abortion fruit of Manaohong Cherry were explored to provide theoretical basis for screening embryo abortion related genes.[Method]The transcriptome sequencing and correlation analysis were performed on samples of aborted cherry embryos at different developmental stages(swollen stage,color changing stage and mature stage)by using Illumina high-throughput sequencing technology.[Result]Clean reads percentages of the samples were above 98%,and mapped reads percentages were above 85%,and Q30 was above 94%.A total of 6 316 differentially expressed genes were screened,among which 1207 differentially expressed genes were found in different comparison groups.The differentially expressed genes annotated to GO were involved in molecular functions,cellular components,biological processes,with the most involvement in biological processes.The differentially expressed genes annotated to the KEGG pathway were involved in 18 branches of the five major pathways,respectively environmental information processing,biological systems,genetic information processing,cellular processes and metabolism.There were more differentially expressed genes annotated to the carbohydrate metabolism branch.A total of 1 806 differentially expressed genes were predicted as transcription factors,and a total of 57 transcription factor families annotated,with the bHLH family being the most abundant.[Conclusion]The gene expression differences between the swollen,color changing and mature stages of embryo abortion fruit of Manaohong Cherry are significant,while the gene expression differences between the color changing and mature stages become smaller.Therefore,embryo abortion related genes can be screened for functional verification before fruit color changing.关键词
玛瑙红樱桃/胚败育/转录组测序/生育期/差异表达基因Key words
Manaohong Cherry/embryo abortion/transcriptome sequencing/reproductive period/differentially expressed gene分类
农业科技引用本文复制引用
宋莎,江旭升,金吉林,张绿萍,张敏..玛瑙红樱桃不同发育时期胚败育果实转录组分析[J].贵州农业科学,2025,53(1):76-82,7.基金项目
贵州省科技计划项目"'玛瑙红'樱桃胚败育下的坐果机制研究"(黔科合基础-ZK[2022]一般233),"贵州玛瑙红等特色樱桃优异种质资源挖掘及保存研究"(黔科合支撑[2022]一般092) （黔科合基础-ZK[2022]一般233）
