吉首大学学报（自然科学版）2024，Vol.45Issue(6)：24-30,7.DOI:10.13438/j.cnki.jdzk.2024.06.004
湘西龙山百合总黄酮的纯化工艺及抑菌活性
Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity
摘要
Abstract
Through comparing the static adsorption-desorption performance of 7 kinds of macroporous resins,the best macroporous resin with high adsorption rate and desorption rate was selected.The opti-mal purification process of Lilium lancifolium by macroporous resin was selected by single factor experi-ment and orthogonal experiment,and the total flavonoid content and antibacterial activity before and af-ter purification were determined.The results showed that the macroporous resin with high adsorption and desorption performance was AB-8.The optimal purification process was as follows:the total fla-vonoid concentration of the feed solution was 0.076 4 g/L,the pH value was 4,the adsorption time was 8 hours,the solvent volume fraction was 60％,the solvent volume was 120 mL,and the desorption time was 10 hours.The total flavonoid content after purification was 11.55％,which was 5.3 times that of the purification before.The inhibition zone diameter of the purified total flavonoid was larger than that of the total flavonoid before purification,and the inhibitory effect on Staphylococcus aureus was higher than that on Escherichia coli.关键词
龙山百合/大孔吸附树脂/总黄酮纯化/抑菌活性Key words
Lilium lancifolium thunb/macroporous adsorption resin/total flavone purification/antibacte-rial activity分类
医药卫生引用本文复制引用
朱蓝萍,袁姣,袁洁盈,尹欣联,刘英,成江,姚姝凤..湘西龙山百合总黄酮的纯化工艺及抑菌活性[J].吉首大学学报（自然科学版）,2024,45(6):24-30,7.基金项目
湖南省自然科学基金区域联合基金(2024JJ7409) （2024JJ7409）
吉首大学2021年引进人员资助研究项目 （）
吉首大学药学院创新性研究项目(2022年立项) （2022年立项）
