吉首大学学报（自然科学版）2024，Vol.45Issue(6)：31-36,6.DOI:10.13438/j.cnki.jdzk.2024.06.005
开口箭根状茎中总皂苷的提取工艺及抑菌活性
Extraction Process and Antibacterial Activity of Total Saponins from Rhizomes of Rohdea chinensis
摘要
Abstract
The total saponin was isolated from Rohdea chinensis rhizome through the ethanol water bath heating extraction method.The influences of ethanol concentration,liquid to material ratio,extraction time and extraction temperature on the yield rate of the total saponin were investigated.On the basis of single factor test,the orthogonal test was used to optimize the extraction process.The optimal extraction conditions of total saponins from the rhizome of Rohdea chinensis are as follows:material-liquid ratio 1∶30(g∶mL),ethanol concentration 60％,extraction time 60 min,and extraction temperature 80 ℃.Un-der these extraction conditions,the total saponin yield of Rohdea chinensis was 17.46％.The results of the antibacterial activity test showed that the total saponins in the rhizome of the Rohdea chinensis had inhibitory effects on Escherichia coli,Staphylococcus aureus,Candida albicans,and the inhibitory activi-ty on Staphylococcus aureus was the best,with the diameter of the inhibition circle 12 mm at a concen-tration of 5 g/L.关键词
开口箭根状茎/总皂苷/提取工艺/抑菌活性/正交试验Key words
rhizomes of Rohdea chinensis/total saponins/extraction process/antibacterial activity/orthog-onal test分类
农业科技引用本文复制引用
郭炜,毛嘉怡,徐亮,卿笃江,成江,姚姝凤..开口箭根状茎中总皂苷的提取工艺及抑菌活性[J].吉首大学学报（自然科学版）,2024,45(6):31-36,6.基金项目
湖南省大学生创新创业训练计划项目(S202310531078) （S202310531078）
吉首大学2021年引进人员资助研究项目 （）
吉首大学校级科研项目(JDX22022) （JDX22022）
