吉首大学学报（自然科学版）2024，Vol.45Issue(6)：37-43,7.DOI:10.13438/j.cnki.jdzk.2024.06.006
玉竹多糖的提取工艺及体外抗氧化活性
Optimization of Extraction Process and Vitro Antioxidant Activity of Total Polysaccharides from Polygonatum odoratum
摘要
Abstract
The extraction process and antioxidant activity of total polysaccharide from the rhizome of Po-lygonatum odoratum were evaluated.The total polysaccharide was isolated from Polygonatum odoratum rhizome through ultrasound-assisted water bath heating extraction method.The influences of material-liquid ratio,ultrasonic power,ultrasonic extraction temperature on the extraction rate of the total poly-saccharide were investigated.Based on the results of single-factor experiments,the response surface ex-periments give the optimal extraction conditions of total polysaccharide from the rhizome of Polygona-tum odoratum∶material-liquid ratio 1∶15(g∶mL),ultrasonic power 300 W,extraction time 60 min,and ultrasonic extraction temperature 60 ℃.Under these extraction conditions,the total polysaccharide yield of Polygonatum odoratum was 13.58％.The results of the antioxidant activity test showed that when the mass concentration of Polygonatum odoratum polysaccharide was 2.75 g/L,the scavenging rates of Polygonatum odoratum polysaccharide on DPPH radicals and OH radicals were 34.18％and 62.04％,respectively.关键词
玉竹/多糖/提取工艺/抗氧化活性/响应面法Key words
Polygonatum odoratum/polysaccharide/extraction process/antioxidant activity/response sur-face method分类
医药卫生引用本文复制引用
张燕,章扬,陈嘉蕊,姚姝凤,成江..玉竹多糖的提取工艺及体外抗氧化活性[J].吉首大学学报（自然科学版）,2024,45(6):37-43,7.基金项目
国家大学生创新创业训练计划项目(202210531011) （202210531011）
吉首大学大学生创新创业训练计划项目(JDCX2023925) （JDCX2023925）
吉首大学药学院创新性研究项目(2022年立项) （2022年立项）
